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Immunosenescence

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Cover of 'Immunosenescence'

Table of Contents

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    Book Overview
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    Chapter 1 Isolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation
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    Chapter 2 Flow Cytometry Analysis of NK Cell Phenotype and Function in Aging.
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    Chapter 3 Flow Cytometric Identification of Fibrocytes in the Human Circulation.
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    Chapter 4 Experimental Approaches to Tissue Injury and Repair in Advanced Age.
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    Chapter 5 Multicolor Digital Flow Cytometry in Human Translational Immunology
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    Chapter 6 Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates.
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    Chapter 7 Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry.
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    Chapter 8 Imaging Immunosenescence.
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    Chapter 9 Activation-Induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza Vaccine
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    Chapter 10 Analyzing the Effect of Aging on CD8+ T-Cell Phenotype Using Flow Cytometry
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    Chapter 11 Immunosenecence
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    Chapter 12 Assays for Monitoring Macroautophagy Activity in T cells.
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    Chapter 13 Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides, and Protein Conformation in Biological Tissues
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    Chapter 14 Monitoring the DNA Damage Response at Dysfunctional Telomeres
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    Chapter 15 Single-Cell Analysis of T-Cell Receptor αβ Repertoire.
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    Chapter 16 Immunosenecence
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    Chapter 17 Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-Throughput DNA Sequencing.
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    Chapter 18 Discovery of Novel microRNAs in Aging Caenorhabditis elegans.
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    Chapter 19 Analysis of DNA Methylation by Pyrosequencing.
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    Chapter 20 ERRATUM
Attention for Chapter 14: Monitoring the DNA Damage Response at Dysfunctional Telomeres
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Chapter title
Monitoring the DNA Damage Response at Dysfunctional Telomeres
Chapter number 14
Book title
Immunosenescence
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2963-4_14
Pubmed ID
Book ISBNs
978-1-4939-2962-7, 978-1-4939-2963-4
Authors

Rekha Rai, Sandy Chang, Rai, Rekha, Chang, Sandy

Abstract

Telomeres are repetitive DNA repeats that cap the ends of all eukaryotic chromosomes. Their proper maintenance is essential for genomic stability and cellular viability. Dysfunctional telomeres could arise through natural attrition of telomeric DNA or due to the removal of shelterin components. These uncapped chromosomal ends are recognized as DSBs by the DDR pathway, leading to the accumulation of DNA damage sensors at telomeres. The association of these DDR proteins with dysfunctional telomeres forms telomere dysfunction induced DNA damage foci (TIFs). Detection of TIFs at telomeres provides an opportunity to quantify the extent of telomere dysfunction and monitor downstream DNA damage signaling pathways. Here we describe a method for the detection of TIFs using a fluorescent in situ hybridization (FISH) approach.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 50%
Student > Bachelor 1 25%
Student > Master 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 50%
Medicine and Dentistry 1 25%
Engineering 1 25%