Chapter title |
Assays for Monitoring Macroautophagy Activity in T cells.
|
---|---|
Chapter number | 12 |
Book title |
Immunosenescence
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2963-4_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2962-7, 978-1-4939-2963-4
|
Authors |
Botbol, Yair, Macian, Fernando, Yair Botbol, Fernando Macian |
Abstract |
Autophagy is an essential catabolic process that regulates a diverse array of functions by targeting cellular components for degradation by lysosomes. Studies in mammalian cells have shown that the regulation of autophagy is highly complex and optimization of experimental approaches to analyze this process needs to be developed for each model studied. This chapter provides an overview of two of the most commonly used ways to monitor autophagy activity in T cell. It involves description of common techniques, namely Western blot and cell immunostaining, giving specific recommendations for working with T cells and monitoring macroautophagy. We also discuss the analysis required for correct interpretation of the results and quantification of macroautophagy activity. |
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Members of the public | 2 | 100% |
Mendeley readers
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Unknown | 3 | 100% |
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Student > Doctoral Student | 1 | 33% |
Unknown | 2 | 67% |
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Agricultural and Biological Sciences | 1 | 33% |
Unknown | 2 | 67% |