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Immunosenescence

Overview of attention for book
Cover of 'Immunosenescence'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Isolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation
  3. Altmetric Badge
    Chapter 2 Flow Cytometry Analysis of NK Cell Phenotype and Function in Aging.
  4. Altmetric Badge
    Chapter 3 Flow Cytometric Identification of Fibrocytes in the Human Circulation.
  5. Altmetric Badge
    Chapter 4 Experimental Approaches to Tissue Injury and Repair in Advanced Age.
  6. Altmetric Badge
    Chapter 5 Multicolor Digital Flow Cytometry in Human Translational Immunology
  7. Altmetric Badge
    Chapter 6 Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates.
  8. Altmetric Badge
    Chapter 7 Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry.
  9. Altmetric Badge
    Chapter 8 Imaging Immunosenescence.
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    Chapter 9 Activation-Induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza Vaccine
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    Chapter 10 Analyzing the Effect of Aging on CD8+ T-Cell Phenotype Using Flow Cytometry
  12. Altmetric Badge
    Chapter 11 Immunosenecence
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    Chapter 12 Assays for Monitoring Macroautophagy Activity in T cells.
  14. Altmetric Badge
    Chapter 13 Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides, and Protein Conformation in Biological Tissues
  15. Altmetric Badge
    Chapter 14 Monitoring the DNA Damage Response at Dysfunctional Telomeres
  16. Altmetric Badge
    Chapter 15 Single-Cell Analysis of T-Cell Receptor αβ Repertoire.
  17. Altmetric Badge
    Chapter 16 Immunosenecence
  18. Altmetric Badge
    Chapter 17 Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-Throughput DNA Sequencing.
  19. Altmetric Badge
    Chapter 18 Discovery of Novel microRNAs in Aging Caenorhabditis elegans.
  20. Altmetric Badge
    Chapter 19 Analysis of DNA Methylation by Pyrosequencing.
  21. Altmetric Badge
    Chapter 20 ERRATUM
Attention for Chapter 12: Assays for Monitoring Macroautophagy Activity in T cells.
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  • Good Attention Score compared to outputs of the same age and source (65th percentile)

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Chapter title
Assays for Monitoring Macroautophagy Activity in T cells.
Chapter number 12
Book title
Immunosenescence
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2963-4_12
Pubmed ID
Book ISBNs
978-1-4939-2962-7, 978-1-4939-2963-4
Authors

Botbol, Yair, Macian, Fernando, Yair Botbol, Fernando Macian

Abstract

Autophagy is an essential catabolic process that regulates a diverse array of functions by targeting cellular components for degradation by lysosomes. Studies in mammalian cells have shown that the regulation of autophagy is highly complex and optimization of experimental approaches to analyze this process needs to be developed for each model studied. This chapter provides an overview of two of the most commonly used ways to monitor autophagy activity in T cell. It involves description of common techniques, namely Western blot and cell immunostaining, giving specific recommendations for working with T cells and monitoring macroautophagy. We also discuss the analysis required for correct interpretation of the results and quantification of macroautophagy activity.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 1 33%
Unknown 2 67%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 33%
Unknown 2 67%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 October 2015.
All research outputs
#15,297,057
of 22,829,683 outputs
Outputs from Methods in molecular biology
#5,299
of 13,125 outputs
Outputs of similar age
#208,065
of 353,131 outputs
Outputs of similar age from Methods in molecular biology
#341
of 997 outputs
Altmetric has tracked 22,829,683 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,125 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,131 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 997 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.