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Mendeley readers
| Chapter title |
Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homoeologous Chromosomes
|
|---|---|
| Chapter number | 4 |
| Book title |
Plant Cytogenetics
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3622-9_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3620-5, 978-1-4939-3622-9
|
| Authors |
Araceli Fominaya, Yolanda Loarce, Juan M. González, Esther Ferrer, Fominaya, Araceli, Loarce, Yolanda, González, Juan M., Ferrer, Esther |
| Abstract |
Tyramide signal amplification (TSA) fluorescence in situ hybridization (FISH) has been shown as a valuable molecular tool for visualizing specific amplified DNA sequences in chromosome preparations. This chapter describes how to perform TSA-FISH, paying special interest to its two critical steps: probe generation and metaphase plate generation. The potential of physically mapping 12S-globulin sequences by TSA-FISH as a means of identifying homeology among chromosome regions of Avena species was tested and is discussed. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 33% |
| Student > Ph. D. Student | 1 | 17% |
| Student > Doctoral Student | 1 | 17% |
| Student > Bachelor | 1 | 17% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Engineering | 1 | 17% |
| Unknown | 1 | 17% |