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Plant Cytogenetics

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Attention for Chapter 12: The Chromosome Microdissection and Microcloning Technique
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Chapter title
The Chromosome Microdissection and Microcloning Technique
Chapter number 12
Book title
Plant Cytogenetics
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3622-9_12
Pubmed ID
Book ISBNs
978-1-4939-3620-5, 978-1-4939-3622-9
Authors

Ying-Xin Zhang, Chuan-Liang Deng, Zan-Min Hu, Zhang, Ying-Xin, Deng, Chuan-Liang, Hu, Zan-Min

Abstract

Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of probes for chromosome painting, and the localization and cloning of important genes. Here, we describe a modified technique to microdissect a single chromosome, paint individual chromosomes, and construct single-chromosome DNA libraries.

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Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 25%
Student > Bachelor 1 25%
Student > Doctoral Student 1 25%
Unknown 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 50%
Agricultural and Biological Sciences 1 25%
Unknown 1 25%