| Chapter title |
Construction of BAC Libraries from Flow-Sorted Chromosomes
|
|---|---|
| Chapter number | 11 |
| Book title |
Plant Cytogenetics
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3622-9_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3620-5, 978-1-4939-3622-9
|
| Authors |
Jan Šafář, Hana Šimková, Jaroslav Doležel, Šafář, Jan, Šimková, Hana, Doležel, Jaroslav |
| Abstract |
Cloned DNA libraries in bacterial artificial chromosome (BAC) are the most widely used form of large-insert DNA libraries. BAC libraries are typically represented by ordered clones derived from genomic DNA of a particular organism. In the case of large eukaryotic genomes, whole-genome libraries consist of a hundred thousand to a million clones, which make their handling and screening a daunting task. The labor and cost of working with whole-genome libraries can be greatly reduced by constructing a library derived from a smaller part of the genome. Here we describe construction of BAC libraries from mitotic chromosomes purified by flow cytometric sorting. Chromosome-specific BAC libraries facilitate positional gene cloning, physical mapping, and sequencing in complex plant genomes. |
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