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DNA Electrophoresis

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Cover of 'DNA Electrophoresis'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 DNA Electrophoresis: Historical and Theoretical Perspectives
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    Chapter 2 DNA Electrophoresis
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    Chapter 3 Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-Dimensional (2D) Native-Native Agarose Gel Electrophoresis.
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    Chapter 4 Analysis of DNA Structures from Eukaryotic Cells by Two-Dimensional Native–Native DNA Agarose Gel Electrophoresis
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    Chapter 5 Directionality of Replication Fork Movement Determined by Two-Dimensional Native–Native DNA Agarose Gel Electrophoresis
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    Chapter 6 Native/Denaturing Two-Dimensional DNA Electrophoresis and Its Application to the Analysis of Recombination Intermediates
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    Chapter 7 Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis.
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    Chapter 8 A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-quantitation of DNA Single-Strand Breaks
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    Chapter 9 Denaturing Gradient Gel Electrophoresis (DGGE)
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    Chapter 10 Polyacrylamide Temperature Gradient Gel Electrophoresis
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    Chapter 11 Separation of DNA Oligonucleotides Using Denaturing Urea PAGE
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    Chapter 12 Pulsed-Field Gel Electrophoresis of Bacterial Chromosomes
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    Chapter 13 Resolution of Budding Yeast Chromosomes Using Pulsed-Field Gel Electrophoresis
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    Chapter 14 Analysis of DNA Damage via Single-Cell Electrophoresis
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    Chapter 15 Fluorescence In Situ Hybridization on Electrophoresed Cells to Detect Sequence Specific DNA Damage
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    Chapter 16 Analysis of DNA-Protein Interactions Using PAGE: Band-Shift Assays
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    Chapter 17 Assaying Cooperativity of Protein–DNA Interactions Using Agarose Gel Electrophoresis
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    Chapter 18 DNA Bending by Proteins: Utilizing Plasmid pBendAT as a Tool
  20. Altmetric Badge
    Chapter 19 Using PCR Coupled to PAGE for Detection and Semiquantitative Evaluation of Telomerase Activity
Attention for Chapter 7: Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis.
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Chapter title
Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis.
Chapter number 7
Book title
DNA Electrophoresis
Published in
Methods in molecular biology, January 2013
DOI 10.1007/978-1-62703-565-1_7
Pubmed ID
Book ISBNs
978-1-62703-564-4, 978-1-62703-565-1
Authors

Jorge B. Schvartzman, María-Luisa Martínez-Robles, Pablo Hernández, Dora B. Krimer

Abstract

Two-dimensional (2D) agarose gel electrophoresis is nowadays one of the best methods available to analyze DNA molecules with different masses and shapes. The possibility to use nicking enzymes and intercalating agents to change the twist of DNA during only one or in both runs, improves the capacity of 2D gels to discern molecules that apparently may look alike. Here we present protocols where 2D gels are used to understand the structure of DNA molecules and its dynamics in living cells. This knowledge is essential to comprehend how DNA topology affects and is affected by all the essential functions that DNA is involved in: replication, transcription, repair and recombination.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 38%
Student > Master 3 23%
Student > Bachelor 2 15%
Student > Ph. D. Student 1 8%
Unknown 2 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 54%
Agricultural and Biological Sciences 5 38%
Unknown 1 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 August 2013.
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#3,560,880
of 4,507,280 outputs
Outputs from Methods in molecular biology
#1,971
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Outputs of similar age
#72,702
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Outputs of similar age from Methods in molecular biology
#18
of 43 outputs
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