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DNA Electrophoresis

Overview of attention for book
Cover of 'DNA Electrophoresis'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 DNA Electrophoresis: Historical and Theoretical Perspectives
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    Chapter 2 DNA Electrophoresis
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    Chapter 3 Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-Dimensional (2D) Native-Native Agarose Gel Electrophoresis.
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    Chapter 4 Analysis of DNA Structures from Eukaryotic Cells by Two-Dimensional Native–Native DNA Agarose Gel Electrophoresis
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    Chapter 5 Directionality of Replication Fork Movement Determined by Two-Dimensional Native–Native DNA Agarose Gel Electrophoresis
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    Chapter 6 Native/Denaturing Two-Dimensional DNA Electrophoresis and Its Application to the Analysis of Recombination Intermediates
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    Chapter 7 Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis.
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    Chapter 8 A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-quantitation of DNA Single-Strand Breaks
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    Chapter 9 Denaturing Gradient Gel Electrophoresis (DGGE)
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    Chapter 10 Polyacrylamide Temperature Gradient Gel Electrophoresis
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    Chapter 11 Separation of DNA Oligonucleotides Using Denaturing Urea PAGE
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    Chapter 12 Pulsed-Field Gel Electrophoresis of Bacterial Chromosomes
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    Chapter 13 Resolution of Budding Yeast Chromosomes Using Pulsed-Field Gel Electrophoresis
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    Chapter 14 Analysis of DNA Damage via Single-Cell Electrophoresis
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    Chapter 15 Fluorescence In Situ Hybridization on Electrophoresed Cells to Detect Sequence Specific DNA Damage
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    Chapter 16 Analysis of DNA-Protein Interactions Using PAGE: Band-Shift Assays
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    Chapter 17 Assaying Cooperativity of Protein–DNA Interactions Using Agarose Gel Electrophoresis
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    Chapter 18 DNA Bending by Proteins: Utilizing Plasmid pBendAT as a Tool
  20. Altmetric Badge
    Chapter 19 Using PCR Coupled to PAGE for Detection and Semiquantitative Evaluation of Telomerase Activity
Attention for Chapter 11: Separation of DNA Oligonucleotides Using Denaturing Urea PAGE
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Chapter title
Separation of DNA Oligonucleotides Using Denaturing Urea PAGE
Chapter number 11
Book title
DNA Electrophoresis
Published in
Methods in molecular biology, July 2013
DOI 10.1007/978-1-62703-565-1_11
Pubmed ID
Book ISBNs
978-1-62703-564-4, 978-1-62703-565-1
Authors

Fiona Flett, Heidrun Interthal, Flett, Fiona, Interthal, Heidrun

Editors

Makovets, Svetlana

Abstract

Denaturing urea polyacrylamide gel electrophoresis (PAGE) allows the separation of linear single-stranded DNA molecules based on molecular weight. This method can be used to analyze or purify short synthesized DNA oligonucleotides or products from enzymatic reactions.In this chapter we describe how to prepare and how to run these high concentration polyacrylamide gels. We detail how to transfer a gel onto Whatman paper and how to dry it. Radiolabelled oligonucleotides are visualized by PhosphorImager technology.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 32%
Student > Ph. D. Student 4 21%
Student > Bachelor 3 16%
Student > Master 2 11%
Student > Postgraduate 1 5%
Other 0 0%
Unknown 3 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 26%
Biochemistry, Genetics and Molecular Biology 4 21%
Chemistry 2 11%
Unspecified 1 5%
Immunology and Microbiology 1 5%
Other 3 16%
Unknown 3 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 November 2013.
All research outputs
#5,314,518
of 7,088,931 outputs
Outputs from Methods in molecular biology
#2,534
of 4,905 outputs
Outputs of similar age
#88,663
of 142,732 outputs
Outputs of similar age from Methods in molecular biology
#9
of 27 outputs
Altmetric has tracked 7,088,931 research outputs across all sources so far. This one is in the 13th percentile – i.e., 13% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,905 research outputs from this source. They receive a mean Attention Score of 1.7. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 142,732 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.