| Chapter title |
Detection and Quantification of Vascular Endothelial Growth Factor Receptor Tyrosine Kinases in Primary Human Endothelial Cells
|
|---|---|
| Chapter number | 4 |
| Book title |
VEGF Signaling
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2917-7_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2916-0, 978-1-4939-2917-7, 978-1-4939-2916-0, 978-1-4939-2917-7
|
| Authors |
Gareth W. Fearnley, Stephen B. Wheatcroft, Sreenivasan Ponnambalam, Fearnley, Gareth W., Wheatcroft, Stephen B., Ponnambalam, Sreenivasan |
| Abstract |
Proteins differ widely in their pattern of expression depending on organism, tissue, and regulation in response to changing conditions. In the mammalian vasculature, the endothelium responds to vascular endothelial growth factors (VEGFs) via membrane-bound receptor tyrosine kinases (VEGFRs) to modulate many aspects of vascular physiology including vasculogenesis, angiogenesis, and blood pressure. Studies on VEGFR biology are thus dependent on detecting expression levels in different cell types and evaluating how changes in protein levels correlate with changing conditions including circulating VEGF levels. Here, we present a robust immunoblot-based protocol for detecting and quantifying VEGFRs in human endothelial cells. Using internal and external standards, we can rapidly evaluate receptor copy number and assess how this is altered in response to the cellular environment. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 2 | 22% |
| Student > Master | 2 | 22% |
| Professor > Associate Professor | 1 | 11% |
| Unknown | 4 | 44% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Physics and Astronomy | 1 | 11% |
| Social Sciences | 1 | 11% |
| Unknown | 5 | 56% |