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VEGF Signaling

Overview of attention for book
Cover of 'VEGF Signaling'

Table of Contents

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    Book Overview
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    Chapter 1 VEGF Splicing and the Role of VEGF Splice Variants: From Physiological-Pathological Conditions to Specific Pre-mRNA Splicing.
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    Chapter 2 Detection and Quantification of VEGF Isoforms by ELISA
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    Chapter 3 Quantitation of Circulating Neuropilin-1 in Human, Monkey, Mouse, and Rat Sera by ELISA.
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    Chapter 4 Detection and Quantification of Vascular Endothelial Growth Factor Receptor Tyrosine Kinases in Primary Human Endothelial Cells
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    Chapter 5 Induction of VEGF Secretion in Cardiomyocytes by Mechanical Stretch
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    Chapter 6 Chromatin Immunoprecipitation Assay: Examining the Interaction of NFkB with the VEGF Promoter.
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    Chapter 7 An Overview of VEGF-Mediated Signal Transduction
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    Chapter 8 Identification of Receptor Tyrosine Kinase Inhibitors Using Cell Surface Biotinylation and Affinity Isolation
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    Chapter 9 VEGF Signaling
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    Chapter 10 In Vitro Angiogenesis Assays
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    Chapter 11 Chemotactic Migration of Endothelial Cells Towards VEGF-A 165
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    Chapter 12 Vasculogenesis and Angiogenesis in VEGF Receptor-1 Deficient Mice
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    Chapter 13 The Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study Angiogenesis
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    Chapter 14 VEGF Gene Transfer to the Utero-Placental Circulation of Pregnant Guinea Pigs to Enhance Fetal Growth
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    Chapter 15 VEGF Gene Transfer to the Utero-Placental Circulation of Pregnant Sheep to Enhance Fetal Growth
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    Chapter 16 Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System.
Attention for Chapter 16: Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System.
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Chapter title
Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System.
Chapter number 16
Book title
VEGF Signaling
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2917-7_16
Pubmed ID
26285757
Book ISBNs
978-1-4939-2916-0, 978-1-4939-2917-7
Authors

Yin, Linlin, Jao, Li-En, Chen, Wenbiao, Linlin Yin, Li-En Jao, Wenbiao Chen

Abstract

Several strategies have been developed to generate targeted gene disruptions in zebrafish.Here we developed a simple targeted gene inactivation strategy in zebrafish using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system. By injecting two simple in vitro-synthesized components [Cas9 mRNA and single guide (sgRNA)] into one-cell-stage embryos, mutations of the target gene could be efficiently generated. We used a codon-optimized version of Cas9 to improve its translation efficiency in zebrafish. In addition, we designed a cloning-free strategy to facilitate the synthesis of sgRNA. The system allows biallelic inactivation of multiple genes simultaneously by co-injecting a mix of sgRNAs with a single Cas9 construct. This flexible strategy of gene inactivation provides an efficient way to interrogate gene functions and genetic interactions in zebrafish.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 35 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 26%
Student > Ph. D. Student 4 11%
Other 2 6%
Student > Doctoral Student 2 6%
Student > Bachelor 2 6%
Other 8 23%
Unknown 8 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 29%
Agricultural and Biological Sciences 9 26%
Neuroscience 3 9%
Medicine and Dentistry 2 6%
Computer Science 1 3%
Other 2 6%
Unknown 8 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2015.
All research outputs
#20,288,585
of 22,824,164 outputs
Outputs from Methods in molecular biology
#9,915
of 13,124 outputs
Outputs of similar age
#295,840
of 353,117 outputs
Outputs of similar age from Methods in molecular biology
#636
of 997 outputs
Altmetric has tracked 22,824,164 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,124 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,117 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 997 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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