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Stem Cells and Good Manufacturing Practices

Overview of attention for book
Cover of 'Stem Cells and Good Manufacturing Practices'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 100 Scalable Ex Vivo Expansion of Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Stirred Culture Systems
  3. Altmetric Badge
    Chapter 101 GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation
  4. Altmetric Badge
    Chapter 102 Culture of Human Limbal Epithelial Stem Cells on Tenon’s Fibroblast Feeder-Layers: A Translational Approach
  5. Altmetric Badge
    Chapter 103 Standard Operating Procedure for the Good Manufacturing Practice-Compliant Production of Human Bone Marrow Mesenchymal Stem Cells
  6. Altmetric Badge
    Chapter 104 Media Fill for Validation of a Good Manufacturing Practice-Compliant Cell Production Process
  7. Altmetric Badge
    Chapter 105 cGMP-Compliant Transportation Conditions for a Prompt Therapeutic Use of Marrow Mesenchymal Stromal/Stem Cells
  8. Altmetric Badge
    Chapter 106 Scalable Expansion of Human-Induced Pluripotent Stem Cells in Xeno-Free Microcarriers
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    Chapter 112 GMP-Compliant Human Adipose Tissue-Derived Mesenchymal Stem Cells for Cellular Therapy
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    Chapter 115 Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting
  11. Altmetric Badge
    Chapter 116 Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions
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    Chapter 117 Bioreactor Expansion of Human Mesenchymal Stem Cells According to GMP Requirements
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    Chapter 118 The Suspension Culture of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks
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    Chapter 122 Xeno-Free Culture of Human Periodontal Ligament Stem Cells.
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    Chapter 123 Labeling Pluripotent Stem Cell-Derived Neural Progenitors with Iron Oxide Particles for Magnetic Resonance Imaging.
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    Chapter 124 Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs
  17. Altmetric Badge
    Chapter 125 Production of Good Manufacturing Practice-Grade Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells for Therapeutic Use
  18. Altmetric Badge
    Chapter 164 Using the Quantum Cell Expansion System for the Automated Expansion of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells
  19. Altmetric Badge
    Chapter 175 Therapeutic Mesenchymal Stromal Cells: Where We Are Headed
Attention for Chapter 100: Scalable Ex Vivo Expansion of Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Stirred Culture Systems
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Chapter title
Scalable Ex Vivo Expansion of Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Stirred Culture Systems
Chapter number 100
Book title
Stem Cells and Good Manufacturing Practices
Published in
Methods in molecular biology, July 2014
DOI 10.1007/7651_2014_100
Pubmed ID
25063496
Book ISBNs
978-1-4939-2434-9, 978-1-4939-2435-6
Authors

Joana G. Carmelo, Ana Fernandes-Platzgummer, Joaquim M. S. Cabral, Cláudia Lobato da Silva, Carmelo, Joana G., Fernandes-Platzgummer, Ana, Cabral, Joaquim M. S., Silva, Cláudia Lobato da

Abstract

The clinical demand for human mesenchymal stem/stromal cells (MSC) drives the need for reproducible, cost-effective, and good manufacturing practices (GMP)-compliant ex vivo expansion protocols. Bioprocess engineering strategies, namely controlled stirred bioreactor systems combined with the use of xenogeneic(xeno)-free materials, provide proper tools to develop and optimize cell manufacturing for the rapid expansion of human MSC for cellular therapies. Herein we describe a microcarrier-based stirred culture system operating under xeno-free conditions using a controlled stirred-tank bioreactor for an efficient and controlled ex vivo expansion of human MSC. This culture platform can be applied to MSC from different human sources, as well as different microcarriers and xeno-free medium formulations.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Australia 1 3%
Switzerland 1 3%
Canada 1 3%
Unknown 30 91%

Demographic breakdown

Readers by professional status Count As %
Student > Master 9 27%
Student > Ph. D. Student 6 18%
Researcher 4 12%
Student > Doctoral Student 2 6%
Other 2 6%
Other 2 6%
Unknown 8 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 27%
Biochemistry, Genetics and Molecular Biology 6 18%
Engineering 4 12%
Chemical Engineering 2 6%
Arts and Humanities 1 3%
Other 2 6%
Unknown 9 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 August 2014.
All research outputs
#18,375,478
of 22,759,618 outputs
Outputs from Methods in molecular biology
#7,870
of 13,089 outputs
Outputs of similar age
#163,447
of 228,763 outputs
Outputs of similar age from Methods in molecular biology
#25
of 70 outputs
Altmetric has tracked 22,759,618 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 228,763 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 70 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.

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