Virus-Derived Nanoparticles for Advanced Technologies
Springer New York
Chapter title |
CCMV-Based Enzymatic Nanoreactors
|
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Chapter number | 16 |
Book title |
Virus-Derived Nanoparticles for Advanced Technologies
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7808-3_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7806-9, 978-1-4939-7808-3
|
Authors |
Mark V. de Ruiter, Rindia M. Putri, Jeroen J. L. M. Cornelissen, Ruiter, Mark V. de, Putri, Rindia M., Cornelissen, Jeroen J. L. M. |
Abstract |
Protein-based nanoreactors are generated by encapsulating an enzyme inside the capsid of the cowpea chlorotic mottle virus (CCMV). Here, three different noncovalent methods are described to efficiently incorporate enzymes inside the capsid of these viral protein cages. The methods are based on pH, leucine zippers, and electrostatic interactions respectively, as a driving force for encapsulation. The methods are exclusively described for the enzymes horseradish peroxidase, glucose oxidase, and Pseudozyma antarctica lipase B, but they are also applicable for other enzymes. |
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