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Salmonella

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Cover of 'Salmonella'

Table of Contents

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    Book Overview
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    Chapter 1 Luminex® Multiplex Bead Suspension Arrays for the Detection and Serotyping of Salmonella spp.
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    Chapter 2 Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle
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    Chapter 3 Determination of Antimicrobial Resistance in Salmonella spp.
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    Chapter 4 Salmonella
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    Chapter 5 A Method to Introduce an Internal Tag Sequence into a Salmonella Chromosomal Gene
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    Chapter 6 Salmonella
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    Chapter 7 Detection of Antimicrobial (Poly)Peptides with Acid Urea Polyacrylamide Gel Electrophoresis Followed by Western Immunoblot
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    Chapter 8 Detecting Non-typhoid Salmonella in Humans by Enzyme-Linked Immunosorbent Assays (ELISAs): Practical and Epidemiological Aspects
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    Chapter 9 Study of the Stn Protein in Salmonella ; A Regulator of Membrane Composition and Integrity
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    Chapter 10 Development of a Bacterial Nanoparticle Vaccine
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    Chapter 11 Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium Membranes Designed for Combination Bacteriotherapy Against Tumors
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    Chapter 12 Applications of Microscopy in Salmonella Research
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    Chapter 13 Live Cell Imaging of Intracellular Salmonella enterica
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    Chapter 14 In Vitro Modeling of Gallbladder-Associated Salmonella spp. Colonization.
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    Chapter 15 Salmonella phages and prophages: genomics, taxonomy, and applied aspects.
Attention for Chapter 13: Live Cell Imaging of Intracellular Salmonella enterica
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Chapter title
Live Cell Imaging of Intracellular Salmonella enterica
Chapter number 13
Book title
Salmonella
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1625-2_13
Pubmed ID
Book ISBNs
978-1-4939-1624-5, 978-1-4939-1625-2
Authors

Alexander Kehl, Michael Hensel

Abstract

During the intracellular phase of the pathogenic lifestyle, Salmonella enterica massively alters the endosomal system of its host cells. Two hallmarks are the remodeling of phagosomes into the Salmonella-containing vacuole (SCV) as a replicative niche, and the formation of tubular structures, such as Salmonella-induced filaments (SIFs). To study the dynamics and the fate of these Salmonella-specific compartments, live cell imaging (LCI) is a method of choice. In this chapter, we compare currently used microscopy techniques and focus on considerations and requirements specific for LCI. Detailed protocols for LCI of Salmonella infection with either confocal laser scanning microscopy (CLSM) or spinning disk confocal microscopy (SDCM) are provided.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Other 1 33%
Professor > Associate Professor 1 33%
Unspecified 1 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 67%
Unspecified 1 33%