Chapter title |
Expanding the Utility of FUCCI Reporters Using FACS-Based Omics Analysis
|
---|---|
Chapter number | 214 |
Book title |
Embryonic Stem Cell Protocols
|
Published in |
Methods in molecular biology, April 2015
|
DOI | 10.1007/7651_2015_214 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2953-5, 978-1-4939-2954-2
|
Authors |
James Chappell, Ben Boward, Stephen Dalton, Chappell, James, Boward, Ben, Dalton, Stephen |
Abstract |
The FUCCI indicator system is a powerful tool for spatio-temporal analysis of the cell cycle, but its utility has been restricted so far to a limited range of applications. Here, we describe how to establish and validate the FUCCI system in murine pluripotent stem cells (PSCs) and describe the utility of transgenic FUCCI mice. We then describe how the FUCCI system can be used to generate material for a wide-range of omics-based applications in conjunction with FACS isolation of cells. This significantly broadens the potential applications of FUCCI reporters for studying the molecular basis of development and disease. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 29% |
Researcher | 5 | 18% |
Student > Bachelor | 3 | 11% |
Student > Master | 2 | 7% |
Professor | 1 | 4% |
Other | 2 | 7% |
Unknown | 7 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 13 | 46% |
Agricultural and Biological Sciences | 2 | 7% |
Environmental Science | 1 | 4% |
Unspecified | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Other | 1 | 4% |
Unknown | 9 | 32% |