Chapter title |
In vitro analysis of cleavage and polyadenylation in Arabidopsis.
|
---|---|
Chapter number | 8 |
Book title |
Polyadenylation in Plants
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2175-1_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2174-4, 978-1-4939-2175-1
|
Authors |
Hongwei Zhao, Qingshun Quinn Li, Zhao, Hongwei, Li, Qingshun Quinn |
Abstract |
In eukaryotes, pre-messenger RNA (pre-mRNA) cleavage and polyadenylation is one of the necessary processing steps that produce a mature and functional mRNA. Regulation on pre-mRNA cleavage and polyadenylation affects other processes such as mRNA translocation, stability, and translation. The process of pre-mRNA cleavage and polyadenylation, and its relationship with RNA splicing and translation, have been extensively studied due to its importance in vivo. A successful in vitro system has provided enormous amount of information to the study of cleavage and polyadenylation in the mammalian and yeast systems. Here, we describe an in vitro pre-mRNA cleavage system that faithfully cleaves pre-mRNA substrate using Arabidopsis cell/tissue cultures. |
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