Chapter title |
In Vitro Polarization of T-Helper Cells
|
---|---|
Chapter number | 11 |
Book title |
T-Helper Cells
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-1212-4_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1211-7, 978-1-4939-1212-4
|
Authors |
Julie Rumble, Benjamin M. Segal |
Abstract |
In vitro polarization of CD4(+) T cells along distinct T-helper (Th) lineages is critical for defining the factors and properties that determine the differentiation, stability, and effector functions of each Th subset. Furthermore, polarized cells can be transferred into naïve syngeneic mice to investigate their trafficking patterns and pathological or therapeutic roles in the setting of infection, autoimmunity, and neoplasia. In this chapter, we describe methods for generating and characterizing a spectrum of CD4(+) Th cell lines in vitro. Protocols are provided that use naïve wild-type or T cell receptor (TCR) transgenic CD4+ T cells, or a polyclonal population of primed CD4+ T cells from immunized mice. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 36% |
Student > Ph. D. Student | 2 | 14% |
Student > Bachelor | 1 | 7% |
Student > Doctoral Student | 1 | 7% |
Professor | 1 | 7% |
Other | 0 | 0% |
Unknown | 4 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 6 | 43% |
Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Agricultural and Biological Sciences | 1 | 7% |
Medicine and Dentistry | 1 | 7% |
Unknown | 4 | 29% |