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Cilia

Overview of attention for book
Attention for Chapter 12: Cilia
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Chapter title
Cilia
Chapter number 12
Book title
Cilia
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3789-9_12
Pubmed ID
Book ISBNs
978-1-4939-3787-5, 978-1-4939-3789-9
Authors

Macaluso, Frank P, Perumal, Geoffrey S, Kolstrup, Johan, Satir, Peter, Frank P. Macaluso, Geoffrey S. Perumal, Johan Kolstrup, Peter Satir

Abstract

CLEM (correlated light and electron microscope) imaging is a highly useful technique for examining primary cilia. With CLEM, it is possible to determine the distribution of tagged proteins along the ciliary membrane and axoneme with high precision. Scanning electron microscopy (SEM) permits measurement of ciliary length and orientation in relation to nearby cellular structures in a 3D image; in optimal cases, this can be combined with superresolution microscopy of selected ciliary components as they enter or leave the cilium. This chapter discusses CLEM methods. In the method described in detail, samples are completely processed for sequential fluorescence and SEM observation. This method is ideal for robust antibody localization and minimizes image manipulation in correlating the fluorescent and SEM images. Alternative methods prepare samples for fluorescence imaging followed by processing for SEM then observation in the SEM. This method is ideal for optimal fluorescence imaging, particularly live cell imaging.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 1 33%
Student > Bachelor 1 33%
Researcher 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 100%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2016.
All research outputs
#7,040,799
of 9,238,399 outputs
Outputs from Methods in molecular biology
#3,683
of 7,229 outputs
Outputs of similar age
#169,606
of 238,677 outputs
Outputs of similar age from Methods in molecular biology
#5
of 11 outputs
Altmetric has tracked 9,238,399 research outputs across all sources so far. This one is in the 13th percentile – i.e., 13% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,229 research outputs from this source. They receive a mean Attention Score of 1.9. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 238,677 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.