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Cilia

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Attention for Chapter 7: Cilia
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Chapter title
Cilia
Chapter number 7
Book title
Cilia
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3789-9_7
Pubmed ID
Book ISBNs
978-1-4939-3787-5, 978-1-4939-3789-9
Authors

Wunderlich, Kirsten A, Wolfrum, Uwe, Kirsten A. Wunderlich, Uwe Wolfrum

Abstract

The outer segment of rod and cone photoreceptor cells represents a highly modified primary sensory cilium. It renews on a daily basis throughout lifetime and effective vectorial transport to the cilium is essential for the maintenance of the photoreceptor cell function. Defects in molecules of transport modules lead to severe retinal ciliopathies. We have recently established a fluorescence recovery after photobleaching (FRAP)-based method to monitor molecular trafficking in living rodent photoreceptor cells. We irreversibly bleach the fluorescence of tagged molecules (e.g. eGFP-Rhodopsin) in photoreceptor cells of native vibratome sections through the retina by high laser intensity. In the laser scanning microscope, the recovery of the fluorescent signal is monitored over time and the kinetics of movements of molecules can be quantitatively ascertained.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 50%
Professor > Associate Professor 1 25%
Professor 1 25%
Readers by discipline Count As %
Neuroscience 2 50%
Agricultural and Biological Sciences 1 25%
Biochemistry, Genetics and Molecular Biology 1 25%