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cAMP Signaling

Overview of attention for book
Cover of 'cAMP Signaling'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Simultaneous Assessment of cAMP Signaling Events in Different Cellular Compartments Using FRET-Based Reporters
  3. Altmetric Badge
    Chapter 2 Recording Intracellular cAMP Levels with EPAC-Based FRET Sensors by Fluorescence Lifetime Imaging
  4. Altmetric Badge
    Chapter 3 A Novel Approach Combining Real-Time Imaging and the Patch-Clamp Technique to Calibrate FRET-Based Reporters for cAMP in Their Cellular Microenvironment
  5. Altmetric Badge
    Chapter 4 Structure-Based, In Silico Approaches for the Development of Novel cAMP FRET Reporters
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    Chapter 5 Automated Image Analysis of FRET Signals for Subcellular cAMP Quantification
  7. Altmetric Badge
    Chapter 6 Channel-Based Reporters for cAMP Detection
  8. Altmetric Badge
    Chapter 7 Imaging Sub-plasma Membrane cAMP Dynamics with Fluorescent Translocation Reporters
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    Chapter 8 Adenoviral Transduction of FRET-Based Biosensors for cAMP in Primary Adult Mouse Cardiomyocytes
  10. Altmetric Badge
    Chapter 9 Generation of Transgenic Mice Expressing FRET Biosensors
  11. Altmetric Badge
    Chapter 10 Photoactivatable Adenylyl Cyclases (PACs) as a Tool to Study cAMP Signaling In Vivo: An Overview
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    Chapter 11 Selective Disruption of the AKAP Signaling Complexes.
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    Chapter 12 Screening for Small Molecule Disruptors of AKAP–PKA Interactions
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    Chapter 13 Structure-Based Bacteriophage Screening for AKAP-Selective PKA Regulatory Subunit Variants.
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    Chapter 14 A yeast-based high-throughput screen for modulators of phosphodiesterase activity.
  16. Altmetric Badge
    Chapter 15 Separation of PKA and PKG Signaling Nodes by Chemical Proteomics
  17. Altmetric Badge
    Chapter 16 Development of Computational Models of cAMP Signaling
Attention for Chapter 14: A yeast-based high-throughput screen for modulators of phosphodiesterase activity.
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Chapter title
A yeast-based high-throughput screen for modulators of phosphodiesterase activity.
Chapter number 14
Book title
cAMP Signaling
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2537-7_14
Pubmed ID
25783886
Book ISBNs
978-1-4939-2536-0, 978-1-4939-2537-7
Authors

Ana Santos de Medeiros, Charles S Hoffman, Charles S. Hoffman

Abstract

Cell-based high-throughput screens (HTSs) targeting heterologously expressed proteins in yeast identify compounds that often display relevant biological activity when tested in cell culture. We developed a fission yeast-based HTS to detect small-molecule inhibitors of mammalian cyclic nucleotide phosphodiesterases (PDEs). These screens are carried out in Schizosaccharomyces pombe using a PKA-repressed fbp1-ura4 reporter whose expression due to low PKA activity prevents cells from growing in medium containing the pyrimidine analog 5-fluoro orotic acid (5FOA). We describe here the steps required to construct strains for screening and to optimize conditions for successful screens.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 50%
Unknown 1 50%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 50%
Unknown 1 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 March 2015.
All research outputs
#20,265,771
of 22,796,179 outputs
Outputs from Methods in molecular biology
#9,899
of 13,111 outputs
Outputs of similar age
#295,776
of 353,053 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
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So far Altmetric has tracked 13,111 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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