| Chapter title |
TGF-β Signaling
|
|---|---|
| Chapter number | 25 |
| Book title |
TGF-β Signaling
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2966-5_25 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2965-8, 978-1-4939-2966-5
|
| Authors |
Feng, Zipei, Zi, Zhike, Liu, Xuedong, Zipei Feng, Zhike Zi, Xuedong Liu |
| Abstract |
TGF-β plays an important role in a myriad of cell activities including differentiation, proliferation, and growth arrest. These effects are influenced by the concentration of TGF-β in the surrounding milieu, which is interpreted by mammalian cells and subsequently translated into meaningful signals that guide their proliferation, survival, or death. To predict cellular responses to TGF-ß signaling based on molecular mechanisms, it is important to consider how cells respond to different ligand doses and how variations in ligand exposure impact Smad signaling dynamics and subsequent gene expression. Here we describe methods to measure TGF-β concentration in the environment and approaches to perturb cellular TGF-β exposure to gain a quantitative understanding of signaling dynamics of this pathway. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 31% |
| Student > Ph. D. Student | 3 | 23% |
| Professor | 1 | 8% |
| Student > Master | 1 | 8% |
| Researcher | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 31% |
| Agricultural and Biological Sciences | 3 | 23% |
| Medicine and Dentistry | 2 | 15% |
| Engineering | 1 | 8% |
| Unknown | 3 | 23% |