Chapter title |
Bioreducible Poly(Beta-Amino Ester)s for Intracellular Delivery of SiRNA
|
---|---|
Chapter number | 8 |
Book title |
SiRNA Delivery Methods
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3112-5_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3111-8, 978-1-4939-3112-5
|
Authors |
Kristen L. Kozielski, Jordan J. Green, Kozielski, Kristen L., Green, Jordan J. |
Abstract |
RNA interference (RNAi) is a powerful tool to target and knockdown gene expression in a sequence-specific manner. RNAi can be achieved by the intracellular introduction of SiRNA; however, intracellular SiRNA delivery remains a challenging obstacle. Herein we describe the use of bioreducible nanoparticles formed using poly(beta-amino ester)s (PBAEs) for safe and efficient SiRNA delivery. Methods for polymer synthesis, nanoparticle formation, and SiRNA delivery using these particles are described. A template protocol for nanoparticle screening is presented and can be used to quickly optimize SiRNA delivery for novel applications. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 7% |
Unknown | 13 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 21% |
Student > Ph. D. Student | 3 | 21% |
Student > Postgraduate | 2 | 14% |
Professor > Associate Professor | 1 | 7% |
Student > Bachelor | 1 | 7% |
Other | 0 | 0% |
Unknown | 4 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 5 | 36% |
Engineering | 2 | 14% |
Medicine and Dentistry | 2 | 14% |
Agricultural and Biological Sciences | 1 | 7% |
Chemical Engineering | 1 | 7% |
Other | 1 | 7% |
Unknown | 2 | 14% |