| Chapter title |
Chitosan Nanoparticles for SiRNA Delivery In Vitro
|
|---|---|
| Chapter number | 12 |
| Book title |
SiRNA Delivery Methods
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3112-5_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3111-8, 978-1-4939-3112-5
|
| Authors |
Héloïse Ragelle, Kevin Vanvarenberg, Gaëlle Vandermeulen, Véronique Préat, Ragelle, Héloïse, Vanvarenberg, Kevin, Vandermeulen, Gaëlle, Préat, Véronique |
| Abstract |
RNA interference, the process in which small interfering RNAs (SiRNAs) silence a specific gene and thus inhibit the associated protein, has opened new doors for the treatment of a wide range of diseases. However, efficient delivery of SiRNAs remains a challenge, especially due to their instability in biological environments and their inability to cross cell membranes. To protect and deliver SiRNAs to mammalian cells, a variety of polymeric nanocarriers have been developed. Among them, the polysaccharide chitosan has generated great interests. This derivative of natural chitin is biodegradable and biocompatible, and can complex SiRNAs into nanoparticles on account of its positive charges. However, chitosan presents some limitations that need to be taken into account when designing chitosan/SiRNA nanoparticles. Here, we describe a method to prepare SiRNA/chitosan nanoparticles with high gene silencing efficiency and low cytotoxicity by using the ionic gelation technique. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 41 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 15% |
| Student > Ph. D. Student | 6 | 15% |
| Student > Bachelor | 4 | 10% |
| Student > Doctoral Student | 2 | 5% |
| Student > Postgraduate | 2 | 5% |
| Other | 4 | 10% |
| Unknown | 17 | 41% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 12% |
| Biochemistry, Genetics and Molecular Biology | 4 | 10% |
| Chemical Engineering | 3 | 7% |
| Medicine and Dentistry | 3 | 7% |
| Other | 2 | 5% |
| Unknown | 18 | 44% |