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SiRNA Delivery Methods

Overview of attention for book
Cover of 'SiRNA Delivery Methods'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Synthesis and Conjugation of Small Interfering Ribonucleic Neutral SiRNNs.
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    Chapter 2 Liver-Targeted SiRNA Delivery Using Biodegradable Poly(amide) Polymer Conjugates
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    Chapter 3 SiRNA Delivery Methods
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    Chapter 4 Highly Efficient SiRNA Delivery Mediated by Cationic Helical Polypeptides and Polypeptide-Based Nanosystems
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    Chapter 5 Disulfide-Bridged Cleavable PEGylation of Poly- l -Lysine for SiRNA Delivery
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    Chapter 6 Preparation of a Cyclic RGD: Modified Liposomal SiRNA Formulation for Use in Active Targeting to Tumor and Tumor Endothelial Cells
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    Chapter 7 A Multifunctional Envelope-Type Nano Device Containing a pH-Sensitive Cationic Lipid for Efficient Delivery of Short Interfering RNA to Hepatocytes In Vivo
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    Chapter 8 Bioreducible Poly(Beta-Amino Ester)s for Intracellular Delivery of SiRNA
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    Chapter 9 Preparation of Polyion Complex Micelles Using Block Copolymers for SiRNA Delivery
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    Chapter 10 Delivery of Small Interfering RNAs to Cells via Exosomes.
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    Chapter 11 Dendrimer Nanovectors for SiRNA Delivery.
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    Chapter 12 Chitosan Nanoparticles for SiRNA Delivery In Vitro
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    Chapter 13 Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery
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    Chapter 14 SiRNA In Vivo-Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast.
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    Chapter 15 TLR9-Targeted SiRNA Delivery In Vivo.
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    Chapter 16 Aptamer-MiRNA Conjugates for Cancer Cell-Targeted Delivery.
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    Chapter 17 Method for Confirming Cytoplasmic Delivery of RNA Aptamers.
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    Chapter 18 Hapten-Binding Bispecific Antibodies for the Targeted Delivery of SiRNA and SiRNA-Containing Nanoparticles
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    Chapter 19 Stable Delivery of CCR5-Directed shRNA into Human Primary Peripheral Blood Mononuclear Cells and Hematopoietic Stem/Progenitor Cells via a Lentiviral Vector.
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    Chapter 20 Hepatic Delivery of Artificial Micro RNAs Using Helper-Dependent Adenoviral Vectors.
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    Chapter 21 Intravascular AAV9 Administration for Delivering RNA Silencing Constructs to the CNS and Periphery.
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    Chapter 22 Efficient Gene Suppression in Dorsal Root Ganglia and Spinal Cord Using Adeno-Associated Virus Vectors Encoding Short-Hairpin RNA.
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    Chapter 23 Synthetic SiRNA Delivery: Progress and Prospects.
Attention for Chapter 21: Intravascular AAV9 Administration for Delivering RNA Silencing Constructs to the CNS and Periphery.
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Chapter title
Intravascular AAV9 Administration for Delivering RNA Silencing Constructs to the CNS and Periphery.
Chapter number 21
Book title
SiRNA Delivery Methods
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3112-5_21
Pubmed ID
Book ISBNs
978-1-4939-3111-8, 978-1-4939-3112-5
Authors

Dufour, Brett D, McBride, Jodi L, Brett D. Dufour, Jodi L. McBride, Dufour, Brett D., McBride, Jodi L.

Abstract

Viral vector delivery of RNA silencing constructs, when administered into vasculature, typically results in poor central nervous system (CNS) transduction due to the inability of the vector to cross the blood-brain barrier (BBB). However, adeno-associated virus serotype 9 (AAV9) has the ability to cross the BBB and robustly transduce brain parenchyma and peripheral tissues at biologically meaningful levels when injected intravenously. Recent work by our lab has shown that this method can be used to deliver RNA silencing constructs, resulting in significant reductions in gene expression in multiple brain regions and in peripheral tissues. Here, we outline a method for delivery of AAV9 vectors expressing RNA interference (RNAi) constructs that lead to robust simultaneous transduction of mouse peripheral tissues and the CNS following a single injection into the jugular vein. Additionally, we outline methods for necropsy and immunofluorescence to detect AAV9 transduction patterns in the rodent CNS following a vascular delivery.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 33%
Unspecified 1 17%
Student > Bachelor 1 17%
Student > Ph. D. Student 1 17%
Unknown 1 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 33%
Veterinary Science and Veterinary Medicine 1 17%
Pharmacology, Toxicology and Pharmaceutical Science 1 17%
Unspecified 1 17%
Unknown 1 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2015.
All research outputs
#15,348,897
of 22,830,751 outputs
Outputs from Methods in molecular biology
#5,344
of 13,126 outputs
Outputs of similar age
#230,863
of 393,540 outputs
Outputs of similar age from Methods in molecular biology
#545
of 1,470 outputs
Altmetric has tracked 22,830,751 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,126 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.