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SiRNA Delivery Methods

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Cover of 'SiRNA Delivery Methods'

Table of Contents

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    Book Overview
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    Chapter 1 Synthesis and Conjugation of Small Interfering Ribonucleic Neutral SiRNNs.
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    Chapter 2 Liver-Targeted SiRNA Delivery Using Biodegradable Poly(amide) Polymer Conjugates
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    Chapter 3 SiRNA Delivery Methods
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    Chapter 4 Highly Efficient SiRNA Delivery Mediated by Cationic Helical Polypeptides and Polypeptide-Based Nanosystems
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    Chapter 5 Disulfide-Bridged Cleavable PEGylation of Poly- l -Lysine for SiRNA Delivery
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    Chapter 6 Preparation of a Cyclic RGD: Modified Liposomal SiRNA Formulation for Use in Active Targeting to Tumor and Tumor Endothelial Cells
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    Chapter 7 A Multifunctional Envelope-Type Nano Device Containing a pH-Sensitive Cationic Lipid for Efficient Delivery of Short Interfering RNA to Hepatocytes In Vivo
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    Chapter 8 Bioreducible Poly(Beta-Amino Ester)s for Intracellular Delivery of SiRNA
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    Chapter 9 Preparation of Polyion Complex Micelles Using Block Copolymers for SiRNA Delivery
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    Chapter 10 Delivery of Small Interfering RNAs to Cells via Exosomes.
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    Chapter 11 Dendrimer Nanovectors for SiRNA Delivery.
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    Chapter 12 Chitosan Nanoparticles for SiRNA Delivery In Vitro
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    Chapter 13 Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery
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    Chapter 14 SiRNA In Vivo-Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast.
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    Chapter 15 TLR9-Targeted SiRNA Delivery In Vivo.
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    Chapter 16 Aptamer-MiRNA Conjugates for Cancer Cell-Targeted Delivery.
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    Chapter 17 Method for Confirming Cytoplasmic Delivery of RNA Aptamers.
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    Chapter 18 Hapten-Binding Bispecific Antibodies for the Targeted Delivery of SiRNA and SiRNA-Containing Nanoparticles
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    Chapter 19 Stable Delivery of CCR5-Directed shRNA into Human Primary Peripheral Blood Mononuclear Cells and Hematopoietic Stem/Progenitor Cells via a Lentiviral Vector.
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    Chapter 20 Hepatic Delivery of Artificial Micro RNAs Using Helper-Dependent Adenoviral Vectors.
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    Chapter 21 Intravascular AAV9 Administration for Delivering RNA Silencing Constructs to the CNS and Periphery.
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    Chapter 22 Efficient Gene Suppression in Dorsal Root Ganglia and Spinal Cord Using Adeno-Associated Virus Vectors Encoding Short-Hairpin RNA.
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    Chapter 23 Synthetic SiRNA Delivery: Progress and Prospects.
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Chapter title
SiRNA Delivery Methods
Chapter number 3
Book title
SiRNA Delivery Methods
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3112-5_3
Pubmed ID
Book ISBNs
978-1-4939-3111-8, 978-1-4939-3112-5
Authors

Dash-Wagh, Suvarna, Langel, Ülo, Ulfendahl, Mats, Suvarna Dash-Wagh, Ülo Langel, Mats Ulfendahl

Abstract

Gene silencing by small interfering RNA (SiRNA) is an attractive therapeutic approach for pathological disorders that targets a specific gene. However, its applications are limited, as naked RNA is rapidly degraded by RNases and is inadequately internalized by the target cells in the body. Several viral and nonviral vectors have been described to improve the delivery of SiRNAs both in cultured cells as well as in vivo. Increasing evidence suggests that cell-penetrating peptides (CPPs) are an efficient, non-cytotoxic tool for intracellular delivery of SiRNA. Recently, a new peptide, PepFect6 (PF6), based system has been described for efficient SiRNA delivery in various cell types. PF6 is an amphipathic stearyl-TP10 peptide carrying a pH titratable trifluoromethylquinoline moiety that facilitate endosomal release. PF6 forms stable non-covalent complexes with SiRNA. Upon internalization, the complexes rapidly escape the endosomal compartment, resulting in robust RNA interference (RNAi) responses. This chapter describes a protocol to use the PF6-nanoparticle technology for SiRNA delivery into organotypic cultures of the inner ear i.e., cochlea. We also highlight different critical points in the peptide/SiRNA complex preparation, transfection and in analyzing the efficacy of PF6-SiRNA associated RNAi response.

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Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 33%
Other 2 33%
Student > Ph. D. Student 1 17%
Researcher 1 17%
Readers by discipline Count As %
Medicine and Dentistry 2 33%
Agricultural and Biological Sciences 1 17%
Pharmacology, Toxicology and Pharmaceutical Science 1 17%
Chemistry 1 17%
Engineering 1 17%
Other 0 0%