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Chapter title |
Immunohistochemical Analysis of Urothelial Carcinoma Tissues for Proliferation and Differentiation Markers
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Chapter number | 4 |
Book title |
Urothelial Carcinoma
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7234-0_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7233-3, 978-1-4939-7234-0
|
Authors |
Michael Rose, Nadine T. Gaisa, Rose, Michael, Gaisa, Nadine T. |
Abstract |
Immunohistochemistry is a standard method in histopathology and enables the localized detection of proteins in histological tissue sections. Specific antibodies are bound to cellular antigens, captured by secondary antibodies or polymers, linked to enzymes and visualized by chromogenic substrates. Here, we describe an automated staining technique for larger slide batches as well as a manual protocol for only few slides using a polymer technique. We focus on differentiation markers, measures of cell proliferation, and therapeutic targets in benign and malignant urothelial tissues. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 1 | 100% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 100% |