Chapter title |
Fabrication of Multielectrode Arrays for Neurobiology Applications
|
---|---|
Chapter number | 12 |
Book title |
Cell-Based Microarrays
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7792-5_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7791-8, 978-1-4939-7792-5
|
Authors |
Mario Malerba, Hayder Amin, Gian N. Angotzi, Alessandro Maccione, Luca Berdondini, Malerba, Mario, Amin, Hayder, Angotzi, Gian N., Maccione, Alessandro, Berdondini, Luca |
Abstract |
Substrate-integrated multielectrode arrays (MEAs) enable multisite, long-term, and label-free sensing and actuation of neuronal electrical signals in reduced cell culture models for network electrophysiology. Conventional, thin-film fabricated passive MEAs typically provide a few tens of electrode sites. New generations of active CMOS-based high-resolution arrays provide the capabilities of simultaneous recordings from thousands of neurons over fields of view of several square millimeters, yet allowing extracellular electrical imaging to be achieved down to the subcellular scale. In turn, such advancement in chip-based electrical readouts can significantly complement recently developed biotechnological and bimolecular techniques for neurobiology applications. Here, we describe (1) a simple method to fabricate passive MEAs and (2) protocols for preparing and growing primary rat hippocampal neuronal cultures and human iPS-derived neurons on MEAs. The aim is to provide reliable protocols for initiating the reader to this technology and for stimulating their further development and experimental use in neurobiology. |
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Members of the public | 1 | 100% |
Mendeley readers
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Student > Ph. D. Student | 4 | 21% |
Student > Doctoral Student | 3 | 16% |
Researcher | 3 | 16% |
Student > Master | 2 | 11% |
Student > Bachelor | 2 | 11% |
Other | 2 | 11% |
Unknown | 3 | 16% |
Readers by discipline | Count | As % |
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Neuroscience | 3 | 16% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Social Sciences | 1 | 5% |
Materials Science | 1 | 5% |
Other | 1 | 5% |
Unknown | 5 | 26% |