| Chapter title |
Electrochemical Detection of Nitric Oxide in Plant Cell Suspensions
|
|---|---|
| Chapter number | 11 |
| Book title |
Plant Nitric Oxide
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3600-7_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3598-7, 978-1-4939-3600-7
|
| Authors |
Sophie Griveau, Angélique Besson-Bard, Fethi Bedioui, David Wendehenne, Griveau, Sophie, Besson-Bard, Angélique, Bedioui, Fethi, Wendehenne, David |
| Abstract |
Nitric oxide is a hydrophobic radical acting as a physiological mediator in plants. Because of its unique properties, the detection of NO in plant tissues and cell suspensions remains a challenge. For this purpose, several techniques are used, each having certain advantages and limitations such as interferences with other species, questionable sensitivity, and/or selectivity or ex situ measurement. Here we describe a very attractive approach for tracking NO in plant cell suspensions using a NO-sensitive homemade platinum/iridium-based electrochemical microsensor. This method constitutes the absolute real-time proof of the production of free NO in physiological conditions. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 29% |
| Professor > Associate Professor | 1 | 14% |
| Student > Postgraduate | 1 | 14% |
| Student > Master | 1 | 14% |
| Unknown | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 2 | 29% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Unknown | 4 | 57% |