| Chapter title |
Plant Nitric Oxide
|
|---|---|
| Chapter number | 3 |
| Book title |
Plant Nitric Oxide
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3600-7_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3598-7, 978-1-4939-3600-7
|
| Authors |
Kumari, Aprajita, Gupta, Alok Kumar, Mishra, Sonal, Wany, Aakanksha, Gupta, Kapuganti Jagadis, Aprajita Kumari, Alok Kumar Gupta, Sonal Mishra, Aakanksha Wany, Kapuganti Jagadis Gupta |
| Abstract |
In plants, nitrate reductase (NR) is a key enzyme that produces nitric oxide (NO) using nitrite as a substrate. Lower plants such as algae are shown to have nitric oxide synthase enzyme and higher plants contain NOS activity but enzyme responsible for NO production in higher plants is subjected to debate. In plant nitric oxide research, it is very important to measure NO very precisely in order to determine its functional role. A significant amount of NO is being scavenged by various cell components. The net NO production depends in production minus scavenging. Here, we describe methods to measure NO from purified NR and inducible nitric oxide synthase from mouse (iNOS), we also describe a method of measure NO scavenging by tobacco cell suspensions and mitochondria from roots. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| South Africa | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Postgraduate | 1 | 33% |
| Student > Doctoral Student | 1 | 33% |
| Unknown | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 1 | 33% |
| Medicine and Dentistry | 1 | 33% |
| Unknown | 1 | 33% |