| Chapter title |
Plant Nitric Oxide
|
|---|---|
| Chapter number | 8 |
| Book title |
Plant Nitric Oxide
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3600-7_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3598-7, 978-1-4939-3600-7
|
| Authors |
Maia, Luisa B, Moura, José J G, Luisa B. Maia, José J. G. Moura, Maia, Luisa B., Moura, José J. G. |
| Abstract |
Electron paramagnetic resonance (EPR) spectroscopy is the ideal methodology to identify radicals (detection and characterization of molecular structure) and to study their kinetics, in both simple and complex biological systems. The very low concentration and short life-time of NO and of many other radicals do not favor its direct detection and spin-traps are needed to produce a new and persistent radical that can be subsequently detected by EPR spectroscopy.In this chapter, we present the basic concepts of EPR spectroscopy and of some spin-trapping methodologies to study NO. The "strengths and weaknesses" of iron-dithiocarbamates utilization, the NO traps of choice for the authors, are thoroughly discussed and a detailed description of the method to quantify the NO formation by molybdoenzymes is provided. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 30% |
| Student > Ph. D. Student | 2 | 20% |
| Student > Bachelor | 2 | 20% |
| Student > Master | 2 | 20% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 5 | 50% |
| Medicine and Dentistry | 2 | 20% |
| Agricultural and Biological Sciences | 1 | 10% |
| Biochemistry, Genetics and Molecular Biology | 1 | 10% |
| Unknown | 1 | 10% |