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Salamanders in Regeneration Research

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Cover of 'Salamanders in Regeneration Research'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Variation in Salamanders: An Essay on Genomes, Development, and Evolution.
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    Chapter 2 Maintaining Eastern Newts ( Notophthalmus viridescens ) for Regeneration Research
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    Chapter 3 Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl
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    Chapter 4 Husbandry of Spanish Ribbed Newts ( Pleurodeles waltl )
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    Chapter 5 Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies
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    Chapter 6 Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH.
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    Chapter 7 Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation
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    Chapter 8 The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration
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    Chapter 9 High-Efficiency Electroporation of the Spinal Cord in Larval Axolotl
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    Chapter 10 Pseudotyped Retroviruses for Infecting Axolotl
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    Chapter 11 Thyroxine-Induced Metamorphosis in the Axolotl (Ambystoma mexicanum).
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    Chapter 12 Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos
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    Chapter 13 In Vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration.
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    Chapter 14 Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas.
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    Chapter 15 Culture and Transfection of Axolotl Cells
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    Chapter 16 Isolation and Culture of Neurospheres from the Adult Newt Brain
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    Chapter 17 Methods for Axolotl Blood Collection, Intravenous Injection, and Efficient Leukocyte Isolation from Peripheral Blood and the Regenerating Limb
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    Chapter 18 Assessing Cardiomyocyte Proliferative Capacity in the Newt Heart and Primary Culture
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    Chapter 19 Long-Term Organ Cultures of Newt Hearts
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    Chapter 20 In Vitro Preparation of Newt Inner Ear Sensory Epithelia as a Model for Repair and Regeneration
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    Chapter 21 Transgenesis in Axolotl ( Ambystoma mexicanum )
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    Chapter 22 Generating and Identifying Axolotls with Targeted Mutations Using Cas9 RNA-Guided Nuclease.
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    Chapter 23 Gene Manipulation for Regenerative Studies Using the Iberian Ribbed Newt, Pleurodeles waltl
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    Chapter 24 Transcriptomics using axolotls.
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    Chapter 25 Sal-Site: Research Resources for the Mexican Axolotl
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    Chapter 26 Data Mining in Newt-Omics, the Repository for Omics Data from the Newt.
Attention for Chapter 14: Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas.
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Chapter title
Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas.
Chapter number 14
Book title
Salamanders in Regeneration Research
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2495-0_14
Pubmed ID
Book ISBNs
978-1-4939-2494-3, 978-1-4939-2495-0
Authors

Patrizia Ferretti, Anoop Kumar

Abstract

Notwithstanding the key importance of in vivo models for understanding patterning and cellular interactions in the regenerating tailed amphibian (salamander) limb, dissection of molecular mechanisms, as in other species, can be greatly aided by robust in vitro models. This chapter focuses on derivation and maintenance of cell lines from adult post-metamorphic salamanders and in particular cells derived from normal and regenerating limbs. We also describe a protocol for nucleofecting newt cells that can be used both to investigate the gene function in short-term studies and to establish stable cell lines.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 36%
Student > Bachelor 2 18%
Researcher 2 18%
Student > Master 2 18%
Professor 1 9%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 64%
Agricultural and Biological Sciences 2 18%
Neuroscience 1 9%
Medicine and Dentistry 1 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 March 2015.
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#13,569,456
of 15,378,024 outputs
Outputs from Methods in molecular biology
#6,336
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Outputs of similar age
#179,730
of 218,082 outputs
Outputs of similar age from Methods in molecular biology
#29
of 38 outputs
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