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Insoluble Proteins

Overview of attention for book
Cover of 'Insoluble Proteins'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins
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    Chapter 2 Overcoming the Solubility Problem in E. coli : Available Approaches for Recombinant Protein Production
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    Chapter 3 Optimization of Culture Parameters and Novel Strategies to Improve Protein Solubility
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    Chapter 4 Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purification
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    Chapter 5 Beyond the Cytoplasm of Escherichia coli : Localizing Recombinant Proteins Where You Want Them
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    Chapter 6 Characterization of Amyloid-Like Properties in Bacterial Intracellular Aggregates
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    Chapter 7 Co-translational Stabilization of Insoluble Proteins in Cell-Free Expression Systems
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    Chapter 8 Functional Expression of Plant Membrane Proteins in Lactococcus lactis
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    Chapter 9 High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range of “Difficult-to-Express” Secretory Recombinant Proteins
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    Chapter 10 Insect Cells–Baculovirus System for the Production of Difficult to Express Proteins
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    Chapter 11 Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins.
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    Chapter 12 Recombinant Glycoprotein Production in Human Cell Lines
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    Chapter 13 Soluble Recombinant Protein Production in Pseudoalteromonas haloplanktis TAC125
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    Chapter 14 A Screening Methodology for Purifying Proteins with Aggregation Problems
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    Chapter 15 Solubilization and Refolding of Inclusion Body Proteins
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    Chapter 16 Bacterial Inclusion Body Purification
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    Chapter 17 Characterization of Intracellular Aggresomes by Fluorescent Microscopy
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    Chapter 18 Dialysis: A Characterization Method of Aggregation Tendency
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    Chapter 19 Applications of Mass Spectrometry to the Study of Protein Aggregation
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    Chapter 20 Insoluble Protein Assemblies Characterized by Fourier Transform Infrared Spectroscopy
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    Chapter 21 Insoluble Protein Characterization by Circular Dichroism (CD) Spectroscopy and Nuclear Magnetic Resonance (NMR)
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    Chapter 22 Methods for Characterization of Protein Aggregates
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    Chapter 23 Predicting the Solubility of Recombinant Proteins in Escherichia coli
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    Chapter 24 Insoluble protein applications: the use of bacterial inclusion bodies as biocatalysts.
Attention for Chapter 11: Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins.
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (77th percentile)
  • High Attention Score compared to outputs of the same age and source (86th percentile)

Mentioned by

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1 blog

Citations

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24 Dimensions

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86 Mendeley
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Chapter title
Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins.
Chapter number 11
Book title
Insoluble Proteins
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2205-5_11
Pubmed ID
Book ISBNs
978-1-4939-2204-8, 978-1-4939-2205-5
Authors

Nettleship JE, Watson PJ, Rahman-Huq N, Fairall L, Posner MG, Upadhyay A, Reddivari Y, Chamberlain JM, Kolstoe SE, Bagby S, Schwabe JW, Owens RJ, Joanne E. Nettleship, Peter J. Watson, Nahid Rahman-Huq, Louise Fairall, Mareike G. Posner, Abhishek Upadhyay, Yamini Reddivari, Jonathan M. G. Chamberlain, Simon E. Kolstoe, Stefan Bagby, John W. R. Schwabe, Raymond J. Owens, Nettleship, Joanne E., Watson, Peter J., Rahman-Huq, Nahid, Fairall, Louise, Posner, Mareike G., Upadhyay, Abhishek, Reddivari, Yamini, Chamberlain, Jonathan M. G., Kolstoe, Simon E., Bagby, Stefan, Schwabe, John W. R., Owens, Raymond J.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 1%
Unknown 85 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 17 20%
Student > Master 12 14%
Researcher 11 13%
Student > Ph. D. Student 10 12%
Student > Doctoral Student 5 6%
Other 12 14%
Unknown 19 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 31 36%
Agricultural and Biological Sciences 11 13%
Neuroscience 5 6%
Medicine and Dentistry 3 3%
Computer Science 2 2%
Other 10 12%
Unknown 24 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 October 2015.
All research outputs
#5,891,594
of 22,831,537 outputs
Outputs from Methods in molecular biology
#1,719
of 13,126 outputs
Outputs of similar age
#78,848
of 353,176 outputs
Outputs of similar age from Methods in molecular biology
#132
of 997 outputs
Altmetric has tracked 22,831,537 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 13,126 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,176 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 997 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.