Chapter title |
Nonviral Gene Therapy of the Nervous System: Electroporation
|
---|---|
Chapter number | 22 |
Book title |
Gene Therapy for Neurological Disorders
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3271-9_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3270-2, 978-1-4939-3271-9
|
Authors |
Xue-Feng Ding, Ming Fan, Ding, Xue-Feng, Fan, Ming |
Abstract |
Electroporation has been widely used to efficiently transfer foreign genes into the mammalian central nervous system (CNS), and thus plays an important role in gene therapeutic studies on some brain disorders. A lot of work concerning electroporation is focused on gene transfer into rodent brains. This technique involves an injection of nucleic acids into the brain ventricle or specific area and then applying appropriate electrical field to the injected area. Here, we briefly introduced the advantages and the basic procedures of gene transfer into the rodent brain using electroporation. Better understanding of electroporation in rodent brain may further facilitate gene therapeutic studies on brain disorders. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 20% |
Researcher | 1 | 20% |
Student > Doctoral Student | 1 | 20% |
Student > Master | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 40% |
Immunology and Microbiology | 1 | 20% |
Agricultural and Biological Sciences | 1 | 20% |
Unknown | 1 | 20% |