Chapter title |
Widespread Neuronal Transduction of the Rodent CNS via Neonatal Viral Injection.
|
---|---|
Chapter number | 17 |
Book title |
Gene Therapy for Neurological Disorders
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3271-9_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3270-2, 978-1-4939-3271-9
|
Authors |
Kim, Ji-Yoen, Grunke, Stacy D, Jankowsky, Joanna L, Ji-Yoen Kim, Stacy D. Grunke, Joanna L. Jankowsky, Grunke, Stacy D., Jankowsky, Joanna L. |
Abstract |
The rapid pace of neuroscience research demands equally efficient and flexible methods for genetically manipulating and visualizing selected neurons within the rodent brain. The use of viral vectors for gene delivery saves the time and cost of traditional germline transgenesis and offers the versatility of readily available reagents that can be easily customized to meet individual experimental needs. Here, we present a protocol for widespread neuronal transduction based on intraventricular viral injection of the neonatal mouse brain. Injections can be done either free-hand or assisted by a stereotaxic device to produce lifelong expression of virally delivered transgenes. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
United States | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 29% |
Student > Ph. D. Student | 4 | 24% |
Lecturer | 1 | 6% |
Student > Doctoral Student | 1 | 6% |
Student > Bachelor | 1 | 6% |
Other | 2 | 12% |
Unknown | 3 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 35% |
Neuroscience | 5 | 29% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Engineering | 1 | 6% |
Other | 0 | 0% |
Unknown | 3 | 18% |