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Listeria monocytogenes

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Cover of 'Listeria monocytogenes'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Sampling the Processing Environment for Listeria
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    Chapter 2 Traditional Methods for Isolation of Listeria monocytogenes
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    Chapter 3 Confirmation of Isolates of Listeria by Conventional and Real-Time PCR
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    Chapter 4 Serotype Assignment by Sero-Agglutination, ELISA, and PCR
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    Chapter 5 Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes
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    Chapter 6 Multilocus Sequence Typing (MLST) of Listeria monocytogenes
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    Chapter 7 Ribotyping and Automated Ribotyping of Listeria monocytogenes
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    Chapter 8 Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes
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    Chapter 9 High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray
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    Chapter 10 Analysis of Listeria monocytogenes Subproteomes.
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    Chapter 11 The Listeria Cell Wall and Associated Carbohydrate Polymers
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    Chapter 12 Use of Bacteriophage Cell Wall-Binding Proteins for Rapid Diagnostics of Listeria
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    Chapter 13 Virulence Characterization of Listeria monocytogenes
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    Chapter 14 Internalization Assays for Listeria monocytogenes
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    Chapter 15 Extraction and Analysis of Plasmid DNA from Listeria monocytogenes
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    Chapter 16 Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method
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    Chapter 17 Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes.
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    Chapter 18 Absolute and Relative Gene Expression in Listeria monocytogenes Using Real-Time PCR
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    Chapter 19 Genome Sequencing of Listeria monocytogenes.
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    Chapter 20 Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels
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    Chapter 21 Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers
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    Chapter 22 Vaccination Studies: Detection of a Listeria monocytogenes- Specific T Cell Immune Response Using the ELISPOT Technique
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    Chapter 23 Sampling the Food Processing Environment: Taking Up the Cudgel for Preventive Quality Management in Food Processing Environments
Attention for Chapter 10: Analysis of Listeria monocytogenes Subproteomes.
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Chapter title
Analysis of Listeria monocytogenes Subproteomes.
Chapter number 10
Book title
Listeria monocytogenes
Published in
Methods in molecular biology, May 2014
DOI 10.1007/978-1-4939-0703-8_10
Pubmed ID
Book ISBNs
978-1-4939-0702-1, 978-1-4939-0703-8
Authors

Hébraud M, Michel Hébraud

Abstract

The proteomic approaches have considerably evolved over the past two decades. This opened the doors for larger scale and deeper explorations of cellular physiology. Like for other living organisms, using the tools of proteomics has undoubtedly improved knowledge about the foodborne pathogen Listeria monocytogenes. Among the different technologies and approaches permanently evolving in the field of proteomics, the 2-DE is an analytical separation method of choice to resolve thousands of proteins simultaneously in a single gel, allowing their quantification, the study of their posttranslational modifications and the understanding of their biological function. In this, 2-DE remains a perfectly complementary technique to the new high-throughput techniques such as shotgun proteomics approaches. Moreover, in order to gain in analysis depth and improve knowledge about the target of action and the function of proteins in relation to their subcellular location, it is necessary to explore more specifically the different subcellular proteomes. Thus, the subproteomic analyses became essential and dramatically increased these last years, particularly on proteins secreted into the extracellular milieu, named exoproteome, or on cell envelope proteins (cell wall and membrane proteins) which are involved in the interactions with the surrounding environment. Here, the extraction and separation of L. monocytogenes subproteomes are described based on cell fractionation and 2-DE techniques. This chapter gives a workflow to obtain the exoproteome, the intracellular proteome, the cell wall, and membrane proteomes of the Gram-positive bacterium L. monocytogenes. The different steps of 2-DE technology, composed of a first dimension based on the separation of proteins according to their charge, an equilibration step, then a second dimension based on the separation of proteins according to their mass, and finally the staining of proteins in the gel are detailed. Emerging technologies to extract the exoproteome or the cell surface proteome after enzymatic shaving and to analyze them by shotgun method are also discussed briefly.

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Geographical breakdown

Country Count As %
Germany 1 25%
Unknown 3 75%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 1 25%
Other 1 25%
Student > Master 1 25%
Unknown 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 50%
Computer Science 1 25%
Unknown 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 December 2014.
All research outputs
#18,387,239
of 22,775,504 outputs
Outputs from Methods in molecular biology
#7,870
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Outputs of similar age
#164,258
of 227,433 outputs
Outputs of similar age from Methods in molecular biology
#49
of 129 outputs
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