Super-Resolution Imaging of Nuclear Bodies by STED Microscopy.
Nuclear Bodies and Noncoding RNAs
Methods in molecular biology, January 2015
Yasushi Okada, Shinichi Nakagawa
The sizes of nuclear bodies and other nuclear structures are normally no more than a few hundred nanometers. This size is below the resolution limit of light microscopy and thus requires electron microscopy for direct observation. Recent developments in super-resolution microscopy have extended the resolution of light microscopy to beyond 100 nm. Here, we describe a super-resolution technique, gated STED, for the analysis of the structure of nuclear bodies, with emphasis on the sample preparation and other technical tips that are important to obtain high-quality super-resolution images.
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