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Nuclear Bodies and Noncoding RNAs

Overview of attention for book
Cover of 'Nuclear Bodies and Noncoding RNAs'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Visualization of lncRNA by Single-Molecule Fluorescence In Situ Hybridization
  3. Altmetric Badge
    Chapter 2 Super-Resolution Imaging of Nuclear Bodies by STED Microscopy.
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    Chapter 3 High-Resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale.
  5. Altmetric Badge
    Chapter 4 Time-Lapse Imaging of Nuclear Bodies
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    Chapter 5 Visualization of Nucleic Acids with Synthetic Exciton-Controlled Fluorescent Oligonucleotide Probes
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    Chapter 6 Live CLEM Imaging to Analyze Nuclear Structures at High Resolution
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    Chapter 7 Ultrastructural Analysis of Nuclear Bodies Using Electron Microscopy
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    Chapter 8 Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy.
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    Chapter 9 Genome-Wide Co-Localization Screening of Nuclear Body Components Using a Fluorescently Tagged FLJ cDNA Clone Library.
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    Chapter 10 Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of RNA Targets (CHART).
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    Chapter 11 RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin.
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    Chapter 12 In Situ Dissection of RNA Functional Subunits by Domain-Specific Chromatin Isolation by RNA Purification (dChIRP).
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    Chapter 13 Extracting, Enriching, and Identifying Nuclear Body Sub-Complexes Using Label-Based Quantitative Mass Spectrometry.
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    Chapter 14 Studying RNA-Binding Protein Interactions with Target mRNAs in Eukaryotic Cells: Native Ribonucleoprotein Immunoprecipitation (RIP) Assays
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    Chapter 15 Cross-Linking and Immunoprecipitation of Nuclear RNA-Binding Proteins
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    Chapter 16 Purification of Noncoding RNA and Bound Proteins Using FLAG Peptide-Conjugated Antisense-Oligonucleotides.
  18. Altmetric Badge
    Chapter 17 MMCT-Mediated Chromosome Engineering Technique Applicable to Functional Analysis of lncRNA and Nuclear Dynamics
  19. Altmetric Badge
    Chapter 18 Reconstitution of Nucleocytoplasmic Transport Using Digitonin-Permeabilized Cells
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    Chapter 19 Genome-Wide Analysis of Long Noncoding RNA Turnover.
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    Chapter 20 Knockdown of Nuclear-Retained Long Noncoding RNAs Using Modified DNA Antisense Oligonucleotides.
  22. Altmetric Badge
    Chapter 21 siRNA Screening of Nuclear Proteins
Attention for Chapter 12: In Situ Dissection of RNA Functional Subunits by Domain-Specific Chromatin Isolation by RNA Purification (dChIRP).
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  • Good Attention Score compared to outputs of the same age and source (71st percentile)

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Citations

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Chapter title
In Situ Dissection of RNA Functional Subunits by Domain-Specific Chromatin Isolation by RNA Purification (dChIRP).
Chapter number 12
Book title
Nuclear Bodies and Noncoding RNAs
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2253-6_12
Pubmed ID
Book ISBNs
978-1-4939-2252-9, 978-1-4939-2253-6
Authors

Jeffrey J Quinn, Howard Y Chang, Jeffrey J. Quinn, Howard Y. Chang

Abstract

Here we describe domain-specific chromatin isolation by RNA purification (dChIRP), a technique for dissecting the functional domains of a target RNA in situ. For an RNA of interest, dChIRP can identify domain-level intramolecular and intermolecular RNA-RNA, RNA-protein, and RNA-DNA interactions and maps the RNA's genomic binding sites with higher precision than domain-agnostic methods. We illustrate how this technique has been applied to the roX1 lncRNA to resolve its domain-level architecture, discover its protein- and chromatin-interacting domains, and map its occupancy on the X chromosome.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Unknown 30 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 29%
Researcher 8 26%
Student > Doctoral Student 2 6%
Student > Master 2 6%
Professor 1 3%
Other 1 3%
Unknown 8 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 32%
Biochemistry, Genetics and Molecular Biology 9 29%
Immunology and Microbiology 2 6%
Medicine and Dentistry 1 3%
Neuroscience 1 3%
Other 1 3%
Unknown 7 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2015.
All research outputs
#7,431,346
of 12,378,406 outputs
Outputs from Methods in molecular biology
#2,444
of 8,316 outputs
Outputs of similar age
#126,975
of 268,189 outputs
Outputs of similar age from Methods in molecular biology
#201
of 847 outputs
Altmetric has tracked 12,378,406 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,316 research outputs from this source. They receive a mean Attention Score of 2.1. This one has gotten more attention than average, scoring higher than 66% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 268,189 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 847 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.