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Nuclear Bodies and Noncoding RNAs

Overview of attention for book
Cover of 'Nuclear Bodies and Noncoding RNAs'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Visualization of lncRNA by Single-Molecule Fluorescence In Situ Hybridization
  3. Altmetric Badge
    Chapter 2 Super-Resolution Imaging of Nuclear Bodies by STED Microscopy.
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    Chapter 3 High-Resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale.
  5. Altmetric Badge
    Chapter 4 Time-Lapse Imaging of Nuclear Bodies
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    Chapter 5 Visualization of Nucleic Acids with Synthetic Exciton-Controlled Fluorescent Oligonucleotide Probes
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    Chapter 6 Live CLEM Imaging to Analyze Nuclear Structures at High Resolution
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    Chapter 7 Ultrastructural Analysis of Nuclear Bodies Using Electron Microscopy
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    Chapter 8 Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy.
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    Chapter 9 Genome-Wide Co-Localization Screening of Nuclear Body Components Using a Fluorescently Tagged FLJ cDNA Clone Library.
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    Chapter 10 Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of RNA Targets (CHART).
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    Chapter 11 RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin.
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    Chapter 12 In Situ Dissection of RNA Functional Subunits by Domain-Specific Chromatin Isolation by RNA Purification (dChIRP).
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    Chapter 13 Extracting, Enriching, and Identifying Nuclear Body Sub-Complexes Using Label-Based Quantitative Mass Spectrometry.
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    Chapter 14 Studying RNA-Binding Protein Interactions with Target mRNAs in Eukaryotic Cells: Native Ribonucleoprotein Immunoprecipitation (RIP) Assays
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    Chapter 15 Cross-Linking and Immunoprecipitation of Nuclear RNA-Binding Proteins
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    Chapter 16 Purification of Noncoding RNA and Bound Proteins Using FLAG Peptide-Conjugated Antisense-Oligonucleotides.
  18. Altmetric Badge
    Chapter 17 MMCT-Mediated Chromosome Engineering Technique Applicable to Functional Analysis of lncRNA and Nuclear Dynamics
  19. Altmetric Badge
    Chapter 18 Reconstitution of Nucleocytoplasmic Transport Using Digitonin-Permeabilized Cells
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    Chapter 19 Genome-Wide Analysis of Long Noncoding RNA Turnover.
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    Chapter 20 Knockdown of Nuclear-Retained Long Noncoding RNAs Using Modified DNA Antisense Oligonucleotides.
  22. Altmetric Badge
    Chapter 21 siRNA Screening of Nuclear Proteins
Attention for Chapter 11: RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin.
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (58th percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

Mentioned by

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3 tweeters

Citations

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4 Dimensions

Readers on

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69 Mendeley
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Chapter title
RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin.
Chapter number 11
Book title
Nuclear Bodies and Noncoding RNAs
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2253-6_11
Pubmed ID
Book ISBNs
978-1-4939-2252-9, 978-1-4939-2253-6
Authors

Jesse Engreitz, Eric S Lander, Mitchell Guttman, Eric S. Lander

Abstract

RNA-centric biochemical purification is a general approach for studying the functions and mechanisms of noncoding RNAs. Here, we describe the experimental procedures for RNA antisense purification (RAP), a method for selective purification of endogenous RNA complexes from cell extracts that enables mapping of RNA interactions with chromatin. In RAP, the user cross-links cells to fix endogenous RNA complexes and purifies these complexes through hybrid capture with biotinylated antisense oligos. DNA loci that interact with the target RNA are identified using high-throughput DNA sequencing.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 3%
Unknown 67 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 23 33%
Researcher 16 23%
Student > Bachelor 6 9%
Student > Master 6 9%
Professor > Associate Professor 5 7%
Other 9 13%
Unknown 4 6%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 35 51%
Agricultural and Biological Sciences 22 32%
Neuroscience 3 4%
Immunology and Microbiology 2 3%
Computer Science 1 1%
Other 2 3%
Unknown 4 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 January 2015.
All research outputs
#2,075,006
of 4,692,692 outputs
Outputs from Methods in molecular biology
#685
of 3,383 outputs
Outputs of similar age
#64,117
of 156,008 outputs
Outputs of similar age from Methods in molecular biology
#74
of 401 outputs
Altmetric has tracked 4,692,692 research outputs across all sources so far. This one has received more attention than most of these and is in the 55th percentile.
So far Altmetric has tracked 3,383 research outputs from this source. They receive a mean Attention Score of 1.4. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 156,008 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.
We're also able to compare this research output to 401 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.