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Digital PCR

Overview of attention for book
Cover of 'Digital PCR'

Table of Contents

  1. Altmetric Badge
    Book Overview
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    Chapter 1 Entering the Pantheon of 21 st Century Molecular Biology Tools: A Perspective on Digital PCR
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    Chapter 2 Basic Concepts and Validation of Digital PCR Measurements
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    Chapter 3 Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies–What Does It Mean?
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    Chapter 4 Control Materials and Digital PCR Methods for Evaluation of Circulating Cell-Free DNA Extractions from Plasma
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    Chapter 5 Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events
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    Chapter 6 Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples
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    Chapter 7 Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR
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    Chapter 8 Testing of General and Human-Associated Fecal Contamination in Waters
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    Chapter 9 Analyzing Copy Number Variation with Droplet Digital PCR
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    Chapter 10 Assessing HER2 Amplification in Plasma cfDNA
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    Chapter 11 Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues Using ddPCR: Analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants
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    Chapter 12 Monitoring of Response and Resistance in Plasma of EGFR -Mutant Lung Cancer Using Droplet Digital PCR
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    Chapter 13 Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients
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    Chapter 14 Droplet Digital PCR for Minimal Residual Disease Detection in Mature Lymphoproliferative Disorders
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    Chapter 15 Quantitation of JAK2 V617F Allele Burden by Using the QuantStudio™ 3D Digital PCR System
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    Chapter 16 Novel Multiplexing Strategies for Quantification of Rare Alleles Using ddPCR
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    Chapter 17 Identification and Use of Personalized Genomic Markers for Monitoring Circulating Tumor DNA
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    Chapter 18 Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping
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    Chapter 19 A Universal Droplet Digital PCR Approach for Monitoring of Graft Health After Transplantation Using a Preselected SNP Set
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    Chapter 20 Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci Using Droplet Digital PCR
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    Chapter 21 DNA Methylation Analysis Using Droplet Digital PCR
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    Chapter 22 Simultaneous Quantification of Multiple Alternatively Spliced mRNA Transcripts Using Droplet Digital PCR
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    Chapter 23 Using Droplet Digital PCR to Analyze Allele-Specific RNA Expression
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    Chapter 24 Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCRTM Assays
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    Chapter 25 Quantification of Circulating MicroRNAs by Droplet Digital PCR
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    Chapter 26 Droplet Digital PCR for Absolute Quantification of Extracellular MicroRNAs in Plasma and Serum: Quantification of the Cancer Biomarker hsa-miR-141
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    Chapter 27 Droplet Digital™ PCR Next-Generation Sequencing Library QC Assay
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    Chapter 28 Phasing DNA Markers Using Digital PCR
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    Chapter 29 ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity
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    Chapter 30 Highly Efficient and Reliable DNA Aptamer Selection Using the Partitioning Capabilities of ddPCR: The Hi-Fi SELEX Method
Attention for Chapter 25: Quantification of Circulating MicroRNAs by Droplet Digital PCR
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  • Good Attention Score compared to outputs of the same age and source (73rd percentile)

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Chapter title
Quantification of Circulating MicroRNAs by Droplet Digital PCR
Chapter number 25
Book title
Digital PCR
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7778-9_25
Pubmed ID
29717458
Book ISBNs
978-1-4939-7776-5, 978-1-4939-7778-9
Authors

Manuela Ferracin, Massimo Negrini, Ferracin, Manuela, Negrini, Massimo

Abstract

MicroRNAs (miRNAs) are released in the blood as cell-free molecules either linked to Ago proteins and LDL or enveloped inside exosomes and microvescicles. The amount of specific circulating microRNAs has been discovered to change accordingly to a disease state and to be potentially used as a disease biomarker. Sensitive and accurate methods for circulating microRNA quantification using probe-based or dye-based digital PCR technology have been developed. With a digital PCR system it is possible to obtain the absolute quantification of specific miRNAs, bypassing several issues related to low abundance targets and miRNA normalization. This chapter addresses the workflow and methods for miRNA assessment in biological fluids using EvaGreen-based droplet digital PCR as well as how to analyze and interpret results.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 36 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 14%
Student > Doctoral Student 5 14%
Student > Master 4 11%
Researcher 3 8%
Other 3 8%
Other 6 17%
Unknown 10 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 28%
Agricultural and Biological Sciences 5 14%
Medicine and Dentistry 3 8%
Pharmacology, Toxicology and Pharmaceutical Science 2 6%
Mathematics 1 3%
Other 2 6%
Unknown 13 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 May 2018.
All research outputs
#13,922,133
of 23,079,238 outputs
Outputs from Methods in molecular biology
#3,908
of 13,200 outputs
Outputs of similar age
#227,913
of 442,597 outputs
Outputs of similar age from Methods in molecular biology
#385
of 1,499 outputs
Altmetric has tracked 23,079,238 research outputs across all sources so far. This one is in the 38th percentile – i.e., 38% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,200 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,597 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.

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