Digital PCR

Chapter 1 Entering the Pantheon of 21 st Century Molecular Biology Tools: A Perspective on Digital PCR

Chapter 2 Basic Concepts and Validation of Digital PCR Measurements

Chapter 3 Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies–What Does It Mean?

Chapter 4 Control Materials and Digital PCR Methods for Evaluation of Circulating Cell-Free DNA Extractions from Plasma

Chapter 5 Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events

Chapter 6 Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples

Chapter 8 Testing of General and Human-Associated Fecal Contamination in Waters

Chapter 9 Analyzing Copy Number Variation with Droplet Digital PCR

Chapter 10 Assessing HER2 Amplification in Plasma cfDNA

Chapter 11 Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues Using ddPCR: Analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants

Chapter 12 Monitoring of Response and Resistance in Plasma of EGFR -Mutant Lung Cancer Using Droplet Digital PCR

Chapter 13 Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients

Chapter 14 Droplet Digital PCR for Minimal Residual Disease Detection in Mature Lymphoproliferative Disorders

Chapter 15 Quantitation of JAK2 V617F Allele Burden by Using the QuantStudio™ 3D Digital PCR System

Chapter 16 Novel Multiplexing Strategies for Quantification of Rare Alleles Using ddPCR

Chapter 17 Identification and Use of Personalized Genomic Markers for Monitoring Circulating Tumor DNA

Chapter 18 Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping

Chapter 19 A Universal Droplet Digital PCR Approach for Monitoring of Graft Health After Transplantation Using a Preselected SNP Set

Chapter 20 Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci Using Droplet Digital PCR

Chapter 21 DNA Methylation Analysis Using Droplet Digital PCR

Chapter 22 Simultaneous Quantification of Multiple Alternatively Spliced mRNA Transcripts Using Droplet Digital PCR

Chapter 23 Using Droplet Digital PCR to Analyze Allele-Specific RNA Expression

Chapter 24 Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCRTM Assays

Chapter 25 Quantification of Circulating MicroRNAs by Droplet Digital PCR

Chapter 26 Droplet Digital PCR for Absolute Quantification of Extracellular MicroRNAs in Plasma and Serum: Quantification of the Cancer Biomarker hsa-miR-141

Chapter 27 Droplet Digital™ PCR Next-Generation Sequencing Library QC Assay

Chapter 28 Phasing DNA Markers Using Digital PCR

Chapter 29 ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity

Chapter 30 Highly Efficient and Reliable DNA Aptamer Selection Using the Partitioning Capabilities of ddPCR: The Hi-Fi SELEX Method