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Affilin–Novel Binding Molecules Based on Human γ-B-Crystallin, an All β-Sheet Protein

Overview of attention for article published in Journal of Molecular Biology, September 2007
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (88th percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

Mentioned by

patent
39 patents
wikipedia
2 Wikipedia pages

Citations

dimensions_citation
42 Dimensions

Readers on

mendeley
48 Mendeley
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Title
Affilin–Novel Binding Molecules Based on Human γ-B-Crystallin, an All β-Sheet Protein
Published in
Journal of Molecular Biology, September 2007
DOI 10.1016/j.jmb.2007.06.045
Pubmed ID
Authors

Hilmar Ebersbach, Erik Fiedler, Tanja Scheuermann, Markus Fiedler, Milton T. Stubbs, Carola Reimann, Gabriele Proetzel, Rainer Rudolph, Ulrike Fiedler

Abstract

The concept of novel binding proteins as an alternative to antibodies has undergone rapid development and is now ready for practical use in a wide range of applications. Alternative binding proteins, based on suitable scaffolds with desirable properties, are selected from combinatorial libraries in vitro. Here, we describe an approach using a beta-sheet of human gamma-B-crystallin to generate a universal binding site through randomization of eight solvent-exposed amino acid residues selected according to structural and sequence analyses. Specific variants, so-called Affilin, have been isolated from a phage display library against a variety of targets that differ considerably in size and structure. The isolated Affilin variants can be produced in Escherichia coli as soluble proteins and have a high level of thermodynamic stability. The crystal structures of the human wild-type gamma-B-crystallin and a selected Affilin variant have been determined to 1.7 A and 2.0 A resolution, respectively. Comparison of the two molecules indicates that the human gamma-B-crystallin tolerates amino acid exchanges with no major structural change. We conclude that the intrinsically stable and easily expressed gamma-B-crystallin provides a suitable framework for the generation of novel binding molecules.

Mendeley readers

The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 2%
Uruguay 1 2%
Switzerland 1 2%
Unknown 45 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 24 50%
Researcher 6 13%
Student > Bachelor 6 13%
Other 4 8%
Student > Master 3 6%
Other 5 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 50%
Biochemistry, Genetics and Molecular Biology 12 25%
Chemistry 4 8%
Medicine and Dentistry 2 4%
Linguistics 1 2%
Other 3 6%
Unknown 2 4%

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2021.
All research outputs
#1,825,837
of 17,468,018 outputs
Outputs from Journal of Molecular Biology
#314
of 10,550 outputs
Outputs of similar age
#33,078
of 298,298 outputs
Outputs of similar age from Journal of Molecular Biology
#2
of 67 outputs
Altmetric has tracked 17,468,018 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,550 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 298,298 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 67 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.