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Signal Transduction Immunohistochemistry

Overview of attention for book
Cover of 'Signal Transduction Immunohistochemistry'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies
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    Chapter 2 Selection and Validation of Antibodies for Signal Transduction Immunohistochemistry
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    Chapter 3 An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry
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    Chapter 4 Optimized Protocol to Make Phospho-Specific Antibodies that Work
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    Chapter 5 Methodology and Technology for Stabilization of Specific States of Signal Transduction Proteins
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    Chapter 6 An Enhanced Antigen-Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-Embedded Tissues
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    Chapter 7 Imaging Techniques in Signal Transduction IHC
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    Chapter 8 Practical Considerations of Image Analysis and Quantification of Signal Transduction IHC Staining
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    Chapter 9 Flow Cytometric Analysis of Cell Signaling Proteins
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    Chapter 10 CytoSys: A Tool for Extracting Cell-Cycle-Related Expression Dynamics from Static Data
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    Chapter 11 Signaling Events Initiated by Kappa Opioid Receptor Activation: Quantification and Immunocolocalization Using Phospho-Selective KOR, p38 MAPK, and K IR 3.1 Antibodies
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    Chapter 12 Immunohistochemical Assessment of Signal Transduction and Cell-Cycle Networks in Neural Tumors
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    Chapter 13 Novel Multicolor Immunofluorescence Technique Using Primary Antibodies Raised in the Same Host Species
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    Chapter 14 Activation and Differentiation of Mesenchymal Stem Cells
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    Chapter 15 Double In Situ Detection of Sonic Hedgehog mRNA and pMAPK Protein in Examining the Cell Proliferation Signaling Pathway in Mouse Embryo
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    Chapter 16 Identifying Intracellular Sites of Eicosanoid Lipid Mediator Synthesis with EicosaCell Assays
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    Chapter 17 Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
Attention for Chapter 3: An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry
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Chapter title
An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry
Chapter number 3
Book title
Signal Transduction Immunohistochemistry
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-024-9_3
Pubmed ID
21370024
Book ISBNs
978-1-61779-023-2, 978-1-61779-024-9
Authors

R. Hal Scofield, Biji T. Kurien, Yaser Dorri, Skyler Dillon, Anil Dsouza, Kurien, Biji T., Dorri, Yaser, Dillon, Skyler, Dsouza, Anil, Scofield, R. Hal

Abstract

Despite its overall simplicity, protein blotting or Western blotting has been proven to be a powerful procedure for the immunodetection of proteins, especially those that are of low abundance, following electrophoresis. The usefulness of this procedure stems from its ability to provide simultaneous resolution of multiple immunogenic antigens within a sample for detection by specific antibodies. Protein blotting has evolved greatly since its inception and researchers have a variety of ways and means to carry out this transfer. This procedure is used in combination with other important antibody-based detection methods such as enzyme-linked immunosorbant assay and immunohistochemistry to provide confirmation of results both in research and diagnostic testing. Specificity of antibodies used for immunohistochemistry is of critical importance and therefore Western blot is a "must" to address antibodies' specificity.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 108 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 2 2%
Indonesia 1 <1%
United States 1 <1%
Unknown 104 96%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 22 20%
Student > Master 16 15%
Student > Ph. D. Student 14 13%
Researcher 7 6%
Student > Doctoral Student 6 6%
Other 13 12%
Unknown 30 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 23 21%
Medicine and Dentistry 17 16%
Agricultural and Biological Sciences 15 14%
Chemistry 6 6%
Pharmacology, Toxicology and Pharmaceutical Science 4 4%
Other 13 12%
Unknown 30 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 February 2013.
All research outputs
#20,182,546
of 22,696,971 outputs
Outputs from Methods in molecular biology
#9,829
of 13,046 outputs
Outputs of similar age
#169,911
of 180,381 outputs
Outputs of similar age from Methods in molecular biology
#199
of 230 outputs
Altmetric has tracked 22,696,971 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,046 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 180,381 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 230 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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