Signal Transduction Immunohistochemistry

Chapter 1 Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies

Chapter 2 Selection and Validation of Antibodies for Signal Transduction Immunohistochemistry

Chapter 3 An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry

Chapter 4 Optimized Protocol to Make Phospho-Specific Antibodies that Work

Chapter 5 Methodology and Technology for Stabilization of Specific States of Signal Transduction Proteins

Chapter 6 An Enhanced Antigen-Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-Embedded Tissues

Chapter 7 Imaging Techniques in Signal Transduction IHC

Chapter 8 Practical Considerations of Image Analysis and Quantification of Signal Transduction IHC Staining

Chapter 9 Flow Cytometric Analysis of Cell Signaling Proteins

Chapter 10 CytoSys: A Tool for Extracting Cell-Cycle-Related Expression Dynamics from Static Data

Chapter 11 Signaling Events Initiated by Kappa Opioid Receptor Activation: Quantification and Immunocolocalization Using Phospho-Selective KOR, p38 MAPK, and K IR 3.1 Antibodies

Chapter 12 Immunohistochemical Assessment of Signal Transduction and Cell-Cycle Networks in Neural Tumors

Chapter 13 Novel Multicolor Immunofluorescence Technique Using Primary Antibodies Raised in the Same Host Species

Chapter 14 Activation and Differentiation of Mesenchymal Stem Cells

Chapter 15 Double In Situ Detection of Sonic Hedgehog mRNA and pMAPK Protein in Examining the Cell Proliferation Signaling Pathway in Mouse Embryo

Chapter 16 Identifying Intracellular Sites of Eicosanoid Lipid Mediator Synthesis with EicosaCell Assays

Chapter 17 Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads