Title |
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures
|
---|---|
Published in |
Frontiers in Zoology, August 2009
|
DOI | 10.1186/1742-9994-6-16 |
Pubmed ID | |
Authors |
Eeva M Soininen, Alice Valentini, Eric Coissac, Christian Miquel, Ludovic Gielly, Christian Brochmann, Anne K Brysting, Jørn H Sønstebø, Rolf A Ims, Nigel G Yoccoz, Pierre Taberlet |
Abstract |
In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding technology for plant mixture with traditional microhistological method. We analysed stomach contents of two ecologically important subarctic vole species, Microtus oeconomus and Myodes rufocanus, with the two methods. DNA barcoding was conducted using the P6-loop of the chloroplast trnL (UAA) intron. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 11 | 2% |
Germany | 4 | <1% |
Spain | 4 | <1% |
United Kingdom | 3 | <1% |
Netherlands | 2 | <1% |
China | 2 | <1% |
Canada | 2 | <1% |
Australia | 2 | <1% |
France | 1 | <1% |
Other | 13 | 3% |
Unknown | 452 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 112 | 23% |
Researcher | 110 | 22% |
Student > Master | 67 | 14% |
Student > Bachelor | 47 | 9% |
Student > Doctoral Student | 20 | 4% |
Other | 79 | 16% |
Unknown | 61 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 278 | 56% |
Environmental Science | 62 | 13% |
Biochemistry, Genetics and Molecular Biology | 41 | 8% |
Medicine and Dentistry | 8 | 2% |
Earth and Planetary Sciences | 5 | 1% |
Other | 23 | 5% |
Unknown | 79 | 16% |