Title |
CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo
|
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Published in |
Brazilian Journal of Medical and Biological Research, March 2014
|
DOI | 10.1590/1414-431x20133356 |
Pubmed ID | |
Authors |
X.M. Wang, S.J. Gao, X.F. Guo, W.J. Sun, Z.Q. Yan, W.X. Wang, Y.Q. Xu, D. Lu |
Abstract |
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was upregulated, after RNAi. Therefore, we concluded that lentivirus-vector-mediated RNAi targeting of CIAPIN1 is a potential approach to reverse MDR of breast cancer. In addition, CIAPIN1 may participate in MDR of breast cancer by regulating P-glycoprotein and P53 expression. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 22% |
Librarian | 1 | 11% |
Student > Doctoral Student | 1 | 11% |
Other | 1 | 11% |
Student > Ph. D. Student | 1 | 11% |
Other | 1 | 11% |
Unknown | 2 | 22% |
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Agricultural and Biological Sciences | 1 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
Social Sciences | 1 | 11% |
Earth and Planetary Sciences | 1 | 11% |
Other | 0 | 0% |
Unknown | 2 | 22% |