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Mendeley readers
Attention Score in Context
| Title |
A Kinetic Study of the Effects of Light on Circadian Rhythmicity of the frq Promoter of Neurospora crassa
|
|---|---|
| Published in |
Journal of Biological Rhythms, February 2014
|
| DOI | 10.1177/0748730413517981 |
| Pubmed ID | |
| Authors |
Van D. Gooch, Alicia E. Johnson, Brian J. Bourne, Bradley T. Nix, Jonna A. Maas, Julie A. Fox, Jennifer J. Loros, Luis F. Larrondo, Jay C. Dunlap |
| Abstract |
The role of the frq gene in the Neurospora crassa circadian rhythm has been widely studied, but technical limitations have hindered a thorough analysis of frq circadian expression waveform. Through our experiments, we have shown an improved precision in defining Neurospora's circadian rhythm kinetics using a codon optimized firefly luciferase gene reporter linked to a frq promoter. In vivo examination of this real-time reporter has allowed for a better understanding of the relationship of the light responsive elements of the frq promoter to its circadian feedback components. We provide a detailed phase response curve showing the phase shifts induced by a light pulse applied at different points of the circadian cycle. Using the frq-luc reporter, we have found that a 12-h light:12-h dark cycle (12L:12D) results in a luciferase expression waveform that is more complex and higher in amplitude than that seen in free-running conditions of constant darkness (DD). When using a lighting regime more consistent with solar timing, rather than a square wave pattern, one observes a circadian waveform that is smoother, lower in amplitude, and different in phasing. Using dim light in place of darkness in these experiments also affects the resulting waveform and phasing. Our experiments illustrate Neurospora's circadian kinetics in greater detail than previous methods, providing further insight into the complex underlying biochemical, genetic, and physiological mechanisms underpinning the circadian oscillator. |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 5% |
| Unknown | 20 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 33% |
| Researcher | 6 | 29% |
| Professor > Associate Professor | 2 | 10% |
| Student > Bachelor | 2 | 10% |
| Professor | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 1 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 52% |
| Biochemistry, Genetics and Molecular Biology | 6 | 29% |
| Psychology | 1 | 5% |
| Energy | 1 | 5% |
| Neuroscience | 1 | 5% |
| Other | 1 | 5% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 April 2014.
All research outputs
#20,264,045
of 22,794,367 outputs
Outputs from Journal of Biological Rhythms
#642
of 691 outputs
Outputs of similar age
#266,190
of 307,708 outputs
Outputs of similar age from Journal of Biological Rhythms
#3
of 3 outputs
Altmetric has tracked 22,794,367 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 691 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 17.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 307,708 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one.