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Chapter title |
Immunoblotting Using Radiolabeled Reagents for Detection
|
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Chapter number | 9 |
Book title |
Detection of Blotted Proteins
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Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2718-0_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2717-3, 978-1-4939-2718-0
|
Authors |
Holger Bartsch, Claudia Franke, Michael P. Bachmann, Bartsch, Holger, Franke, Claudia, Bachmann, Michael P. |
Abstract |
Development of immunoblots is commonly performed using enzyme-labeled antibodies which convert soluble substrates into insoluble colored products. A simple, rapid, and sensitive alternative method which produces low background and allows a rapid quantitative evaluation is the use of radiolabeled antibodies or protein A conjugates. Here we describe the use of iodinated secondary antibodies for immunodetection of an autoantigen during HPLC purification. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor > Associate Professor | 1 | 100% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 1 | 100% |