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Reprogramming of primary human Philadelphia chromosome-positive B cell acute lymphoblastic leukemia cells into nonleukemic macrophages

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, March 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Citations

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36 Dimensions

Readers on

mendeley
155 Mendeley
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2 CiteULike
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Title
Reprogramming of primary human Philadelphia chromosome-positive B cell acute lymphoblastic leukemia cells into nonleukemic macrophages
Published in
Proceedings of the National Academy of Sciences of the United States of America, March 2015
DOI 10.1073/pnas.1413383112
Pubmed ID
Authors

James Scott McClellan, Christopher Dove, Andrew J. Gentles, Christine E. Ryan, Ravindra Majeti

Abstract

BCR-ABL1(+) precursor B-cell acute lymphoblastic leukemia (BCR-ABL1(+) B-ALL) is an aggressive hematopoietic neoplasm characterized by a block in differentiation due in part to the somatic loss of transcription factors required for B-cell development. We hypothesized that overcoming this differentiation block by forcing cells to reprogram to the myeloid lineage would reduce the leukemogenicity of these cells. We found that primary human BCR-ABL1(+) B-ALL cells could be induced to reprogram into macrophage-like cells by exposure to myeloid differentiation-promoting cytokines in vitro or by transient expression of the myeloid transcription factor C/EBPα or PU.1. The resultant cells were clonally related to the primary leukemic blasts but resembled normal macrophages in appearance, immunophenotype, gene expression, and function. Most importantly, these macrophage-like cells were unable to establish disease in xenograft hosts, indicating that lineage reprogramming eliminates the leukemogenicity of BCR-ABL1(+) B-ALL cells, and suggesting a previously unidentified therapeutic strategy for this disease. Finally, we determined that myeloid reprogramming may occur to some degree in human patients by identifying primary CD14(+) monocytes/macrophages in BCR-ABL1(+) B-ALL patient samples that possess the BCR-ABL1(+) translocation and clonally recombined VDJ regions.

Twitter Demographics

The data shown below were collected from the profiles of 104 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 155 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 2 1%
Austria 1 <1%
Italy 1 <1%
Germany 1 <1%
United Kingdom 1 <1%
Egypt 1 <1%
China 1 <1%
United States 1 <1%
Unknown 146 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 40 26%
Researcher 27 17%
Student > Bachelor 19 12%
Student > Master 15 10%
Student > Doctoral Student 13 8%
Other 20 13%
Unknown 21 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 53 34%
Biochemistry, Genetics and Molecular Biology 26 17%
Medicine and Dentistry 23 15%
Immunology and Microbiology 8 5%
Engineering 4 3%
Other 11 7%
Unknown 30 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 293. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 March 2018.
All research outputs
#64,122
of 17,593,470 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#1,580
of 89,488 outputs
Outputs of similar age
#970
of 224,264 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#33
of 971 outputs
Altmetric has tracked 17,593,470 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 89,488 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 31.1. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 224,264 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 971 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.