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Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza

Overview of attention for article published in BMC Genomics, March 2015
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Title
Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza
Published in
BMC Genomics, March 2015
DOI 10.1186/s12864-015-1411-x
Pubmed ID
Authors

Caili Li, Dongqiao Li, Fenjuan Shao, Shanfa Lu

Abstract

WRKY proteins comprise a large family of transcription factors and play important regulatory roles in plant development and defense response. The WRKY gene family in Salvia miltiorrhiza has not been characterized. A total of 61 SmWRKYs were cloned from S. miltiorrhiza. Multiple sequence alignment showed that SmWRKYs could be classified into 3 groups and 8 subgroups. Sequence features, the WRKY domain and other motifs of SmWRKYs are largely conserved with Arabidopsis AtWRKYs. Each group of WRKY domains contains characteristic conserved sequences, and group-specific motifs might attribute to functional divergence of WRKYs. A total of 17 pairs of orthologous SmWRKY and AtWRKY genes and 21 pairs of paralogous SmWRKY genes were identified. Maximum likelihood analysis showed that SmWRKYs had undergone strong selective pressure for adaptive evolution. Functional divergence analysis suggested that the SmWRKY subgroup genes and many paralogous SmWRKY gene pairs were divergent in functions. Various critical amino acids contributed to functional divergence among subgroups were detected. Of the 61 SmWRKYs, 22, 13, 4 and 1 were predominantly expressed in roots, stems, leaves, and flowers, respectively. The other 21 were mainly expressed in at least two tissues analyzed. In S. miltiorrhiza roots treated with MeJA, significant changes of gene expression were observed for 49 SmWRKYs, of which 26 were up-regulated, 18 were down-regulated, while the other 5 were either up-regulated or down-regulated at different time-points of treatment. Analysis of published RNA-seq data showed that 42 of the 61 identified SmWRKYs were yeast extract and Ag(+)-responsive. Through a systematic analysis, SmWRKYs potentially involved in tanshinone biosynthesis were predicted. These results provide insights into functional conservation and diversification of SmWRKYs and are useful information for further elucidating SmWRKY functions.

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Mendeley readers

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The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 40 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 28%
Researcher 5 13%
Student > Doctoral Student 3 8%
Professor 2 5%
Professor > Associate Professor 2 5%
Other 5 13%
Unknown 12 30%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 48%
Biochemistry, Genetics and Molecular Biology 7 18%
Chemical Engineering 1 3%
Environmental Science 1 3%
Unknown 12 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 April 2015.
All research outputs
#15,327,280
of 22,796,179 outputs
Outputs from BMC Genomics
#6,689
of 10,648 outputs
Outputs of similar age
#170,619
of 286,345 outputs
Outputs of similar age from BMC Genomics
#193
of 285 outputs
Altmetric has tracked 22,796,179 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 10,648 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
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