L. Ait Mbarek, H. Ait Mouse, N. Elabbadi, M. Bensalah, A. Gamouh, R. Aboufatima, A. Benharref, A. Chait, M. Kamal, A. Dalal, A. Zyad
Abstract
The objective of the present study was to evaluate the in vitro and in vivo anti-cancer effect of Nigella sativa L. seed extracts. The essential oil (IC50 = 0.6%, v/v) and ethyl acetate (IC50 = 0.75%) extracts were more cytotoxic against the P815 cell line than the butanol extract (IC50 = 2%). Similar results were obtained with the Vero cell line. Although all extracts had a comparable cytotoxic effect against the ICO1 cell line, with IC50 values ranging from 0.2 to 0.26% (v/v), tests on the BSR cell line revealed a high cytotoxic effect of the ethyl acetate extract (IC50 = 0.2%) compared to the essential oil (IC50 = 1.2%). These data show that the cytotoxicity of each extract depends on the tumor cell type. In vivo, using the DBA2/P815 (H2d) mouse model, our results clearly showed that the injection of the essential oil into the tumor site significantly inhibited solid tumor development. Indeed, on the 30th day of treatment, the tumor volume of the control animals was 2.5 +/- 0.6 cm(3), whereas the tumor volumes of the essential oil-treated animals were 0.22 +/- 0.1 and 0.16 +/- 0.1 cm(3) when the animals were injected with 30 microL (28.5 mg)/mouse and 50 microL (47.5 mg)/mouse per 48 h (six times), respectively. Interestingly, the administration of the essential oil into the tumor site inhibited the incidence of liver metastasis development and improved mouse survival.
Pharmacology, Toxicology and Pharmaceutical Science
21
9%
Chemistry
18
8%
Other
25
11%
Unknown
64
27%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 May 2020.
All research outputs
#17,248,081
of 26,110,873 outputs
Outputs from Brazilian Journal of Medical and Biological Research
#720
of 1,269 outputs
Outputs of similar age
#78,733
of 89,806 outputs
Outputs of similar age from Brazilian Journal of Medical and Biological Research
#6
of 7 outputs
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