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Role of discoidin domain receptor 1 in dysregulation of collagen remodeling by cyclosporin A.

Overview of attention for article published in International Journal of Biochemistry & Cell Biology, March 2015
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9 Mendeley
Title
Role of discoidin domain receptor 1 in dysregulation of collagen remodeling by cyclosporin A.
Published in
International Journal of Biochemistry & Cell Biology, March 2015
DOI 10.1016/j.biocel.2015.02.019
Pubmed ID
Authors

Staudinger LA, Spano SJ, Lee WS, Coelho N, Moriarty TJ, McCulloch CA

Abstract

The anti-transplant rejection drug cyclosporin A (CsA) causes loss of collagen homeostasis in rapidly remodeling connective tissues, such as human gingiva. As a result of CsA treatment, collagen degradation by fibroblasts is inhibited, which leads to a net increase of tissue collagen and gingival overgrowth. Since fibrillar collagen is the primary ligand for the discoidin domain receptor 1 (DDR1), we hypothesized that CsA perturbs DDR1-associated functions that affect collagen homeostasis. For these experiments, human fibroblasts obtained from gingival explants or mouse 3T3 fibroblasts (wild type, over-expressing DDR1 or DDR1 knockdown) or mouse GD25 cells (expressing DDR1 but null for β1 integrin), were treated with vehicle (dimethyl sulfoxide) or with CsA. The effect of CsA on cell binding to collagen was examined by flow cytometry; cell-mediated collagen remodeling was analyzed with contraction, compaction and migration assays. We found that CsA inhibited cell binding to collagen, internalization of collagen, contraction of collagen gels and cell migration over collagen in a DDR1-dependent manner. CsA also enhanced collagen compaction around cell extensions. Treatment with CsA strongly reduced surface levels of β1 integrins in wild type and DDR1 over-expressing 3T3 cells but did not affect β1 integrin activation or focal adhesion formation. We conclude that CsA inhibition of collagen remodeling is mediated through its effects on both DDR1 and cell surface levels of the β1 integrin.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 1 11%
Unknown 8 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 22%
Researcher 2 22%
Professor 1 11%
Student > Bachelor 1 11%
Student > Master 1 11%
Other 2 22%
Readers by discipline Count As %
Medicine and Dentistry 4 44%
Biochemistry, Genetics and Molecular Biology 2 22%
Pharmacology, Toxicology and Pharmaceutical Science 1 11%
Agricultural and Biological Sciences 1 11%
Immunology and Microbiology 1 11%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2016.
All research outputs
#4,655,579
of 8,636,243 outputs
Outputs from International Journal of Biochemistry & Cell Biology
#641
of 1,167 outputs
Outputs of similar age
#110,860
of 204,261 outputs
Outputs of similar age from International Journal of Biochemistry & Cell Biology
#14
of 28 outputs
Altmetric has tracked 8,636,243 research outputs across all sources so far. This one is in the 27th percentile – i.e., 27% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,167 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 204,261 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.