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Seroprevalence and genetic characterization of Toxoplasma gondii in cancer patients in Anhui Province, Eastern China

Overview of attention for article published in Parasites & Vectors, March 2015
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Title
Seroprevalence and genetic characterization of Toxoplasma gondii in cancer patients in Anhui Province, Eastern China
Published in
Parasites & Vectors, March 2015
DOI 10.1186/s13071-015-0778-5
Pubmed ID
Authors

Lin Wang, Liu-yuan He, De-di Meng, Zhao-wu Chen, He Wen, Gong-si Fang, Qing-li Luo, Kai-quan Huang, Ji-long Shen

Abstract

Recent studies have indicated the predominance of Toxoplasma gondii genotype Chinese 1 in animals in China. However, little is known of the genetic features of the parasite in humans. This study aims to determine the prevalence of anti-T. gondii antibodies based on which the genetic character of the parasite was identified in cancer patients in China. A total of 1014 serum samples with malignant neoplasms were collected from six tertiary-care hospitals (HAUCM, APH, HAMU, XAH, FHH and HBMC) from January, 2012 to August, 2013. Antibodies against T. gondii were examined by enzyme-linked immunosorbent assay (ELISA). Blood samples were subsequently used for PCR assay to detect T. gondii DNA (gra6). The DNA positive samples were subjected to genotyping using a multiplex multilocus nested PCR-RFLP at 10 loci, including sag1, sag2, sag3, btub, gra6, l358, c22-8, c29-2, pk1 and apico. Samples from the patients were anonymous and only data with regard to age and gender was available at sample collection. Overall, 8.38% (85/1014) of the examined patients showed positive antibodies against T. gondii. Among them, 61 (6.02%) were seropositive only for IgG, 16 (1.58%) were only for IgM, and 8 (0.79%) were found to be positive for both IgG and IgM. The seroprevalence of antibodies to Toxoplasma ranged from 5.8% to 11.0%, without regional difference (χ (2) = 4.764, P = 0.445). No significant differences of the positive rates of T. gondii infection were noted in genders (male, 8.96%; female, 7.45%) (χ (2) = 0.707, P = 0.400) and in ages (χ (2) = 1.172, P = 0.947). Of 1014 DNA samples, 36 (3.55%) were positive for T. gondii by nested PCR at gra6 locus and nine gave rise to complete genotyping results. All samples with achieved PCR-RFLP genotyping showed a common genetic character of type Chinese 1 (ToxoDB#9). Seroprevalence of toxoplasmosis in immunosuppressed individuals is rarely reported in China and we presented a positive rate of 8.38% in cancer patients. Toxoplasma genomic DNA genotyping demonstrated a common genetic character of Chinese 1, indicating a possible pathogenic origin of animals in human infection.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Italy 1 2%
Brazil 1 2%
Unknown 43 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 24%
Student > Master 8 18%
Student > Postgraduate 6 13%
Student > Bachelor 5 11%
Researcher 4 9%
Other 8 18%
Unknown 3 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 31%
Medicine and Dentistry 10 22%
Biochemistry, Genetics and Molecular Biology 3 7%
Veterinary Science and Veterinary Medicine 2 4%
Immunology and Microbiology 2 4%
Other 8 18%
Unknown 6 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2015.
All research outputs
#9,968,005
of 12,451,686 outputs
Outputs from Parasites & Vectors
#2,379
of 3,208 outputs
Outputs of similar age
#154,765
of 220,966 outputs
Outputs of similar age from Parasites & Vectors
#9
of 12 outputs
Altmetric has tracked 12,451,686 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,208 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 220,966 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.